Copyright
©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 7, 2005; 11(13): 1991-1994
Published online Apr 7, 2005. doi: 10.3748/wjg.v11.i13.1991
Published online Apr 7, 2005. doi: 10.3748/wjg.v11.i13.1991
Enhancement of leukocyte adhesion after percutaneous irradiation in rats with hepatocellular carcinoma
Sasa-Marcel Maksan, Eduard Schmidt, Eduard Ryschich, Jan Schmidt, Department of Surgery, University of Heidelberg, Germany
Wolfgang Harms, Department of Radiation Therapy, University of Heidelberg, Germany
Author contributions: All authors contributed equally to the work.
Correspondence to: Sasa-Marcel Maksan, MD., Department of General Surgery, University of Mainz, Langenbeckstr. 1, D 55118 Mainz, Germany. maksan@ach.klinik.uni-mainz.de
Telephone: +49-6131-171 Fax: +49-6131-176630
Received: March 27, 2004
Revised: March 28, 2004
Accepted: April 13, 2004
Published online: April 7, 2005
Revised: March 28, 2004
Accepted: April 13, 2004
Published online: April 7, 2005
Abstract
AIM: To evaluate the effects of percutaneous radiation on leukocyte-endothelium interaction (LEI) in experimental hepatocellular carcinoma (HCC).
METHODS: Twelve ACI rats underwent HCC-inoculation, six of which on day 12 received low-dose external radiation and six did not. After 12 h intravital microscopy was performed.
RESULTS: LEI was significantly reduced in tumor tissue. However, irradiation of liver sinusoids and tumor tissue with 6 Gy led to a significant activation of leukocyte adhesion in the tumor with a marked increase of the proinflammatory cytokine TNF-α.
CONCLUSION: The findings indicate that the immunological tumor-endothelial barrier can be overcome by external irradiation.
Keywords: Radiation, Hepatocellular carcinoma, Immune response, Animal model