Published online Sep 26, 2016. doi: 10.4330/wjc.v8.i9.553
Peer-review started: May 16, 2016
First decision: July 5, 2016
Revised: July 15, 2016
Accepted: July 29, 2016
Article in press: August 1, 2016
Published online: September 26, 2016
To determine the optimum resting tension (ORT) for in vitro human pulmonary artery (PA) ring preparations.
Pulmonary arteries were dissected from disease free sections of the resected lung in the operating theatre and tissue samples were directly sent to the laboratory in Krebs-Henseleit solution (Krebs). The pulmonary arteries were then cut into 2 mm long rings. PA rings were mounted in 25 mL organ baths or 8 mL myograph chambers containing Krebs compound (37 °C, bubbled with 21% O2: 5% CO2) to measure changes in isometric tension. The resting tension was set at 1-gram force (gf) with vessels being left static to equilibrate for duration of one hour. Baseline contractile reactions to 40 mmol/L KCl were obtained from a resting tension of 1 gf. Contractile reactions to 40 mmol/L KCl were then obtained from stepwise increases in resting tension (1.2, 1.4, 1.6, 1.8 and 2.0 gf).
Twenty PA rings of internal diameter between 2-4 mm were prepared from 4 patients. In human PA rings incrementing the tension during rest stance by 0.6 gf, up to 1.6 gf significantly augmented the 40 mmol/L KCl stimulated tension. Further enhancement of active tension by 0.4 gf, up to 2.0 gf mitigate the 40 mmol/L KCl stimulated reaction. Both Myograph and the organ bath demonstrated identical conclusions, supporting that the radial optimal resting tension for human PA ring was 1.61 g.
The radial optimal resting tension in our experiment is 1.61 gf (15.78 mN) for human PA rings.
Core tip: Pulmonary artery (PA) vasoconstriction is an important physiological process to regulate blood flow in the lungs but it also manifests in pathological conditions. Different models have been implemented to assess the baseline molecular and cellular functions of pulmonary ailments. However, a great deal of the research was undertaken on animals with little similarity to human tissue. Isolation of human PA and measurement of pulmonary vascular tension are vital to understand the pathophysiology of human pulmonary vessels. The objective behind this research is to assess the underlying resting tension for undertaking studies of the PA rings in humans.