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World J Stem Cells. Oct 26, 2013; 5(4): 98-105
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.98
Published online Oct 26, 2013. doi: 10.4252/wjsc.v5.i4.98
Advances in homology directed genetic engineering of human pluripotent and adult stem cells
Kalpith Ramamoorthi, Donald Curtis, Prashanth Asuri, Department of Bioengineering, Santa Clara University, Santa Clara, CA 95053, United States
Author contributions: All authors have contributed to this article.
Correspondence to: Prashanth Asuri, PhD, Department of Bioengineering, Santa Clara University, 500 El Camino Real, Santa Clara, CA 95053, United States. asurip@scu.edu
Telephone: +1-408-5513005 Fax: +1-408-5545474
Received: August 15, 2013
Revised: September 6, 2013
Accepted: September 14, 2013
Published online: October 26, 2013
Revised: September 6, 2013
Accepted: September 14, 2013
Published online: October 26, 2013
Core Tip
Core tip: This manuscript focuses on the development of novel technologies to precisely alter the human stem cell genome and highlights their implications for both basic and applied stem cell research. Specifically, we discuss the development of two main technologies: molecular engineering of viral vectors and design of artificial endonucleases. We also discuss the merits of combining these complementary approaches and suggest other possible strategies that could be explored to further improve genetic engineering of human stem cells.