⊙研究论文⊙

rhIFN-αb和苦味叶下珠提取物对人肝癌细胞株

PLC/PRF/5产生HBsAg的影响

纪徐淮,秦一中,汪伟业,朱建英,许国铭

第二军医大学长海医院 上海市 200433
项目负责人:纪徐淮
收稿日期:1994-08-08 接受日期:1994-09-24

Effects of rhIFN-αb and extracts of phyllanthus urinaria L. on HBsAg production in PLC/PRF/5 cell line

Xu-Huai Ji, Yi-Zhong Qing, Wei-Ye Wang, Jian-Ying Zhu and Guo-Ming Xu
Changhai Hospital, Second Military Medical University, Shanghai 200433 China

Abstract
AIMS To investigate the therapeutic effect of drugs onchronic hepatitis B virus infection.

METHODS Effects of rhIFN-αb and extracts of phyllanthus urinaria L. on HBsAg production in PLC/PRF/5 human hepatoma cell line were in vestigated in vitro.

RESULTS Decreased HBsAg production was regularly demonstrated 48h  after the treatment of cells with 50-250IU/mL of  rhIFN-αb and 2-4mg/mL phyllanthus urinaria L, especially combining IFN with 10^-5mol/L Ara-A. Treatment of PLC/PRF/5 cells with hydrocortisone acetate (10^-3mol/L) and Ara-A(10^-5mol/L) greatly stimulated HBsAg production. The decrease of extracellular HBsAg excretion seemed to be due to inhibition of intracellular HBsAg formation.

CONCLUSIONS Interferon alfa, in combination with DNA synthesis inhibitors may decrease the HBsAg formation in PLC/PRF/5 cells and a crudeextracts from phyllanthus urinaria L. can also inhibit the HBsAg formation. This cell line is a convenient model for deeper studies of chemotherapy of chronically HBV carriers.

Keywords interferon Ara-Ahepatoma cell hepatitis B virus-phyllanthus urinaria L

Ji XH, Qing YZ, Wang WY, Zhu JY,Xu GM.Effects of rhIFN-αb and extracts of phyllanthus urinaria L. on HBsAg production in PLC/PRF/5 cell line. Xin Xiaohuabingxue Zazhi,1995；3(3)