World J Gastroenterol. 2012 September 21; 18(35): 4801-4810.
Published online 2012 September 21. doi: 10.3748/wjg.v18.i35.4801.
Biliary cysts: Etiology, diagnosis and management
Beata Jabłońska.
Beata Jabłońska, Department of Digestive Tract Surgery, University Hospital of the Medical University of Silesia, 40-752 Katowice, Poland
Author contributions: Jabłońska B wrote this paper.
Correspondence to: Beata Jabłońska, MD, PhD, Department of Digestive Tract Surgery, University Hospital of the Medical University of Silesia, Medyków 14 St, 40-752 Katowice, Poland. bjablonska@poczta.onet.pl
Telephone: +48-32-7894251 Fax: +48-32-7894251
Received January 13, 2012; Revised June 5, 2012; Accepted July 9, 2012;
Abstract
Biliary cysts (BC) are rare dilatations of different parts of a biliary tract. They account for approximately 1% of all benign biliary diseases. BC occur the most frequently in Asian and female populations. They are an important problem for pediatricians, gastroenterologists, radiologists and surgeons. Clinical presentation and management depend on the BC type. Cholangiocarcinoma is the most serious and dangerous BC complication. The other complications associated with BC involve cholelithiasis and hepatolithiasis, cholangitis, acute and chronic pancreatitis, portal hypertension, liver fibrosis and secondary liver cirrhosis and spontaneous cyst perforation. Different BC classifications have been described in the literature. Todani classification dividing BC into five types is the most useful in clinical practice. The early diagnosis and proper treatment are very important, because BC are associated with a risk of carcinogenesis. A malignancy risk increases with the age. Radiological investigations (ultrasonography, computed tomography, endoscopic retrograde cholangiopancreatography and magnetic resonance cholangiopancreatography) play an important role in BC diagnostics. Currently, prenatal diagnosis using ultrasonography is possible. It allows to differentiate biliary disorders in fetals and to perform the early surgical treatment that improves results. In most patients, total cyst excision with Roux-Y hepaticojejunostomy is the treatment of choice. Surgical treatment of BC is associated with high success rate and low morbidity and mortality. The early treatment is associated with a lower number of complications. Patients following BC surgery require permanent and careful postoperative observation using laboratory and imaging investigations because of possibility of biliary anastomosis stricture and biliary cancer in tissue remnant.
Keywords: Biliary cysts, Biliary drainage, Hepaticojejunostomy
INTRODUCTION
Biliary cysts (BC) are rare dilatations of different parts of a biliary tract. They account for approximately 1% of all benign biliary diseases. BC occur the most commonly in Asian populations and in females (with a female:male ratio of 3-4:1). Incidence of this pathology in Asian populations is 1:1000 and it is lower in western countries (1:100 000 to 1:150  000)[1-6]. They are usually a surgical problem of infancy or childhood; however in approximately 20% of cases they are recognized in adults[2]. The early diagnosis and proper treatment are very important, because BC are associated with a risk of carcinogenesis. According to the literature, biliary tract malignancy occurs in 2.5%-28% of patients with BC[3,4]. The risk of biliary cancer increases with the age. It is the lowest in the childhood in the first decade (< 1%)[6]. In the third decade the cancer risk is > 10%[7,8]. According to the literature, cholangiocarcinoma is reported the most frequently at the age of 32 years in patients with BC (about 20 years earlier than in general population)[7]. There are reports of cholangiocarcinoma developing in adult many years after radical BC resection in infant[9]. Cholangiocarcinoma is the most serious and dangerous BC complication. The other complications associated with BC involve cholelithiasis and hepatolithiasis, cholangitis, acute and chronic pancreatitis, portal hypertension, liver fibrosis and secondary liver cirrhosis and spontaneous cyst perforation[8-12]. Nicholl et al[9] noted a higher number of complications in adults compared to children. Therefore, BC constitute an important problem for pediatricians, gastroenterologists, radiologists and surgeons. The role of pediatricians and gastroenterologists is a proper early diagnosis. Radiological investigations play an important role in BC diagnostics. Currently, the prenatal BC diagnosis using ultrasonography (USG) is possible[6,13,14].
Surgery is the treatment of BC. The goal of surgical treatment is to remove a cyst and to reconstruct the proper bile flow to the alimentary tract. In order to achieve this goal, different techniques are used. Roux-Y hepaticojejunostomy is the method of choice in most patients with BC[5-22].
ETIOLOGY AND PATHOGENESIS OF BC
Many theories explain the etiology and pathogenesis of BC[2]. In 1936, Yotsuyanagi supposed that BC arouse from inequality in the cellular proliferation of the biliary tract during the early fetal life[23]. Babbitts’s theory of the “common channel” (1969) is the most widely accepted in the literature. According to this theory, the common cannel is formed by abnormal pancreaticobiliary junction (APBJ) of the pancreatic and bile ducts outside the ampulla of Vater. This condition leads to pancreaticobiliary reflux and mixing of the pancreatic and biliary juices and activations of pancreatic enzymes, because the maximal pressure within the pancreatic duct is two to three timed higher than within a biliary tract. Activated pancreatic enzymes cause inflammation and deterioration of the bile duct wall that leads to biliary dilatation[24]. A number of studies analyzed the levels of amylase within the bile duct. The higher amylase concentrations have been observed in patients with BC compared to the control groups. Also, association between the amylase level, earlier presentation and dysplasia grade in patients with BC has been reported in the literature[25,26]. Therefore, there is a theory, that the pancreaticobiliary reflux leads to inflammation and dysplasia in patients with BC[2,26-29]. APBJ occurs only in 50%-80% patients with BC. Another counterargument to this hypothesis is a fact, that immature neonatal acini do not produce sufficient pancreatic enzymes to explain antenatal BC[2,30,31]. The counterargument supporting Babbitt’s theory is arbitral definition of the long common channel that depends on imaging modality and angles. Therefore, a defined length of a long common channel has been described from 10 mm to 45 mm in the literature. Therefore, APBJ can occur in more patients than radiological investigations show[2]. According to Okada et al[32], long common channel as any pancreaticobiliary junction is defined, if it lies outside of the duodenal wall and thus could result in pancreaticobiliary reflux and mixing. APBJ has been divided into three types by Komi et al[33]: (1) A right-angled union without an accessory pancreatic duct; (2) An acute-angled union without an accessory pancreatic duct; and (3) A right- or acute-angled union with an accessory pancreatic duct.
These types are subdivided into subtypes according to the shape of the common channel. The Komi’s classification is presented in Table 1. The type of BC and clinical presentation can depend on the type of an angle of the anomalous ductal junction. A right-angled union is associated with cystic dilatation of the common bile duct. In patients with this type of union a palpable mass or jaundice as the main sign of disease are observed. An acute-angled union is associated with fusiform dilatation of the common bile duct. Patients with such dilatation usually complain on abdominal pain, and an erroneous diagnosis of acute pancreatitis is occasionally made. Type IB, IIB, and III junctions are associated with a dilated common channel and an accessory pancreatic duct. They are frequently complicated by relapsing pancreatitis leading to chronic pancreatitis[34]. Therefore, it’s important to describe the type of APBJ. The type of APBJ is based on a cholangiopancreatographical picture[33-35]. The other theories regarding BC pathogenesis are less popular. According to Singham et al[2], biliary dilation can be a result of embryologic overproliferation of epithelial cells within solid bile ducts during the fetal life. According to Davenport and Basu, dilations are due to a lower number of neurons and ganglions in patients with BC that have been recognized pathologically. They suggested that round cysts were congenital with distal obstruction due to aganglionosis and proximal dilation similarly to Hirschprung’s disease. In this theory, chronic inflammation and clinical manifestation are caused by bile stasis within dilated bile ducts[2,31,36].
Table 1
Table 1
Types of an anomalous pancreaticobiliary junctions (Komi classification)
CLASSIFICATIONS OF BC
The first classification system for BC was proposed by Alonso-LEJ et al[37] in 1959. This classification involves three BC types: congenital cystic dilatation (the most common), congenital diverticulum of the common bile duct, and congenital choledochocele[37]. In 1977, Todani et al[38,39]modified BC classifications distinguishing five types (Figure 1). The most frequent (more than 90%) is type I cyst. The type IA cyst involves dilatation of the common bile duct, with marked dilatation of part or all of the extrahepatic bile ducts. The type IB cyst involves segmental dilatation of the common bile duct, usually of its most distal part. The type IC cyst involves fusiform dilatation of the common bile duct, along with diffuse, cylindric dilatation of the common hepatic duct and common bile duct. In 2011, Michaelides et al[40] reported and proposed a new BC variant and called it the ID type. In this new variant, apart from the dilatation of the common hepatic and the common bile duct, dilatation of the central portion of the cystic duct was also observed, giving a bicornal configuration to the cyst. The type II cyst is a diverticulum of the common bile duct usually arising laterally but may arise in the pancreatic portion. The type III cyst (which is called a choledochocele) is a cystic dilatation of the intraduodenal portion of the common bile duct. According to some authors, choledochocele is different from other types of BC. In 2010, Ziegler et al[41], compared choledochocele with other types of BC (types I, II, IV and V according to Todani classification). Based on study including 146 patients with BC (46 patients with choledochoceles), they concluded that classifications of choledochal cysts should not include choledochoceles. They reported that patients with choledochoceles differed from patients with other choledochal cysts with respect to age, gender, presentation, pancreatic ductal anatomy, and their management. Patients with cholechoceles were the most frequently male, older, complained with acute pancreatitis rather than jaundice or cholangitis, and were managed with endoscopic therapy. A pancreas divisum and a low risk of malignancy compared to other BC were observed with patients with choledochoceles. The type IV cyst involves dilatation of the intrahepatic and extrahepatic bile ducts. In the type IVA cyst, both the intrahepatic and extrahepatic bile ducts are dilated. The type IVB cyst involves dilatation of multiple segments of the extrahepatic bile ducts. The type V cyst (Caroli’s disease) involves dilatation of one or several segments of the intrahepatic bile ducts. First, it was described by Todd in 1818, but precise definition of this disorder was constructed by Jaques Caroli in 1958[41-45]. There are two descriptions (Caroli’s disease and Caroli’s syndrome) in the literature. Caroli’s syndrome is the more serious condition compared to Caroli’s disease. In Caroli’s disease, cholangitis due to bile stasis within dilated intrahepatic bile ducts, is observed. In Caroli’s syndrome, recurrent bouts of cholangitis due to bile stasis, hepatolithiasis, gallbladder stones lead to liver fibrosis with portal hypertension and liver failure. Caroli’s syndrome can be associated with autosomal recessive polycystic kidney disease caused by a mutation of the PKHD1 gene[46,47].
Figure 1
Figure 1
Figure 1
Types of biliary cysts according to Todani classification. I: Solitary extrahepatic cyst (40%-85%): IA: Common type; IB: Segmental extrahepatic duct dilatation; IC: Diffuse extrahepatic dilatation; II: Extrahepatic diverticulum (2%-3%); III: Choledochocele (1.4%-5.6%); IVA: Multiple extra- and intrahepatic cysts; IVB: Multiple extrahepatic cysts (18%-20%); V: Multiple intrahepatic cysts (Caroli’s disease) (rare).
BC can be associated with the biliary atresia. Three biliary atresia (BA) types have been distinguished in the literature: 1 BA (atresia of the common bile duct), 2 BA (atresia of the common hepatic duct), and 3 BA (atresia of the porta hepatis)[18]. Muise et al[18], concluded that BA associated with BC formed a distinct subtype of BA, characterized by a preponderance of type 1 BA, a relatively good clinical outcome after surgery, and an absence of associated congenital anomalies. In 2004, Visser et al[48] proposed modified classification of BC and distinguished the following BC types: choledochal cyst, choledochal diverticulum, choledochocele, and Caroli’s disease. All BC classifications are presented in Table 2.
Table 2
Table 2
Classifications of biliary cysts
CLINICAL PRESENTATION OF BC
The classic triad of clinical symptoms was described by Alonso-LEJ et al[37] and it involves right hypochondriac pain, palpable abdominal mass and jaundice. All clinical signs are observed only in 20%-30% patients[49]. Two of three clinical symptoms occur in 2/3 patients. The most frequently (in 80% of patients) clinical presentation appears before the age of 10 years[44]. Clinical presentation is different and it depends on the patient’s age. According to the age, patients are divided into two groups: neonatal patients (under 12 mo) and adults (above 13 mo). Prenatally, BC are diagnosed incidentally during prenatal ultrasonography visualized as an intra-abdominal cystic mass[6,13,14]. In neonatal patients, obstructive jaundice and abdominal mass are present the most frequently. Pain, fever and nausea are the most common for adult patients[44]. According to Lopez et al[21], pain is the commonest clinical sign in adults. Recurrent cholangitis and pancreatitis are complications of BC due to pancreaticobiliary reflux[49,50]. The other complications observed commonly in adults are the following: cholangiocarcinoma, cholecystolithiasis and cholecystitis, and liver cirrhosis[5]. Cholecystolithiasis and cholecystitis occur due to bile stasis in patients with BC. The secondary liver cirrhosis leads to different complications associated with portal hypertension such as upper gastrointestinal bleeding, splenomegaly and pancytopenia[49]. Portal hypertension can also occur without liver cirrhosis due to mechanical obstruction of the portal vein by the cyst[48]. An spontaneous BC rupture occurs in about 1%-12% of patients. It is presented as abdominal pain, peritonitis signs and sepsis[49,51]. Patients with choledochocele are usually asymptomatic. The III BC type can be also presented by gastric outlet obstruction symptoms caused by direct obstruction of the duodenal lumen or intussusceptions[44]. It should be highlighted that BC are premalignant disorder. The overall reported risk of cancer is 10%-30% and it increases with age (from 0% in patients aged < 10 years to 75% in patients aged 70-80 years). Nicholl et al[9] reported direct correlation between the patient age and cancer risk: 0 year to 30 years (0%), 31 years to 50 years (19%), and 51 years to 70 years (50%). The histopathological types of cancer are the following: adenocarcinoma (73%-84%), anaplastic carcinoma (10%), undifferentiated cancer (5%-7%), squamous cell carcinoma (5%), and others (1.5%). The locations of cancer are the following: the extrahepatic bile duct (50%-62%), gallbladder (38%-46%), intrahepatic bile ducts (2.5%), the liver (0.7%), and the pancreas (0.7%)[49]. Todani et al[52] reported that 68% of cancers were associated with type-I, 5% type-II, 1.6% type-III, 21% type-IV and 6% type-V BC. Cholangiocarcinoma occurs as a result of chronic inflammation, cell regeneration and DNA breaks, leading to dysplasia[49,52]. The following molecular lesions have been reported in BC during carcinogenesis: microsatellite instability, k-ras mutations, expression of COX-2 and bcl-2, and increased telomerase activity that occur early and involvement of cyclin D1, beta-catenin, DPC-4/Smad4 and p53 that occur later[7].
DIAGNOSTICS OF BC
Laboratory investigations
Laboratory investigations may demonstrate mildly abnormal liver function and cholestasis tests (serum bilirubin, alkaline phosphatase, γ-glutamyltranspeptidase, alanine and aspartate aminotransferases) or amylase values, but these findings are not specific[34,49-56].
Radiological investigations
Imaging investigations are the most useful in BC diagnosis. The following radiological examinations are performed in order to visualize biliary dilatation: ultrasonography (USG) of the abdominal cavity, computed tomography (CT) of the abdominal cavity, and cholangiography[34,49-56].
USG of abdominal cavity
USG is the initial and easy to perform examination. It allows imaging of intrahepatic and extrahepatic bile ducts with measurement of the diameter of common bile duct or common hepatic duct and BC. It demonstrates (with the exception of type-III and type-V cysts) a cystic mass in the right upper quadrant (usually at the porta hepatis) separately from the gallbladder[49].
Use of USG in prenatal diagnosis
USG is very useful in prenatal diagnosis of BC. USG shows BC as a round intra-abdominal cystic mass located in the upper abdominal quadrant. Color Doppler USG shows no significant flow within the mass. Differential diagnosis of the cystic mass in prenatal USG should involve simple hepatic cysts, biliary atresia, ovarian, omental or mesenteric cysts, duodenal or gall bladder duplications, adrenal cysts, renal cysts, dilated loop of bowel, hydronephrotic renal pelvis and situs inversus. Proper early differential diagnosis between BC and another biliary disorder such as BA is very important, because BA needs an immediate surgery[6,13,14]. When the differential diagnosis of a cystic mass in the right upper quadrant is difficult to make by conventional USG alone, ultrasound-guided BC aspiration may serve as an alternative to the prenatal diagnosis of BC in the fetus[57,58]. Tanaka et al[14] compared imaging (USG and CT) and laboratory investigations in BC and BA in order to differentiate these two pathologies. They concluded that patients with biliary disorder smaller than 21 mm, direct bilirubin level higher than 2.5 mg/dL, and total bile acid level higher than 111 µmol/L in the neonatal period were more likely to have BA than BC and needed a surgery as soon as possible before irreversible liver cirrhosis. Okada et al[17] described the differential diagnosis between BC and BA by immunohistological examination using liver biopsy specimens. They indicated that CD56-positive biliary duct cells were present in prenatally diagnosed type-1 cystic BA.
Cholangiography
Proper diagnosis requires demonstration of continuity of the cyst with the biliary tract, because it allows to differentiate BC from other intrabdominal cysts such as pancreatic pseudocysts, echinococcal cysts or biliary cystadenomas[49]. In order to show it, cholangiography is performed. There are the following methods of cholangiography: endoscopic retrograde cholangiopancreatography (ERCP), magnetic resonance-cholangiopancreatography (MRCP), percutaneous transhepatic cholangiography (PTC) and intraoperative cholangiography. These investigations allow to demonstrate the anatomy of biliary tree and define the BC type[49].
ERCP
ERCP is invasive investigation, but it has got therapeutic possibility. It accurately reveals the presence of any associated intraductal pathology or an APBJ. In the a type III BC, it allows to perform a therapeutic papillotomy simultaneously[49].
MRCP
MRCP is a non-invasive procedure compared to ERCP. It is a favoured alternative to ERCP, but it has a lower accuracy in the detection of APBDJ and lacks the therapeutic possibility in case of the type III BC. MRCP, as a non-invasive procedure, is investigation of choice in preoperative imaging of biliary tree[5,49,54-56]. A gadoxetic acid-enhanced MRCP in order to diagnose the biliopancreatic bile reflux and pancreatico-biliary reflux of pancreatic secretions in patients with anomalous union of the pancreatico-biliary duct has been described in the literature. Gadoxetic acid-enhanced MRCP can visualize the physiology of bile excretion, in contrast to conventional T2-weighted MRC which can visualize fluid filled space by heavily T2-weighted and fat-suppressed images. It is associated with specific properties of the gadoxetic acid which is uptaken by hepatocytes. Gadoxetic acid is excreted into the bile ducts that allows visualization of the bile ducts on hepatobiliary phase T1-weighted images[59].
PTC
Preoperative PTC allows to define the proximal extent of biliary dilatation and to use this information in preoperative resection planning[49]. According to Lipsett et al[53], the preoperative placement of a percutaneous ring catheter after cholangiography is useful for the surgeon during the operative procedure and can be used as a stent for the biliary reconstruction.
A technetium-99 HIDA investigation
A technetium-99 HIDA investigation is recommended to visualize the continuity BC with bile ducts. This investigation shows an initial area of photopenia within BC, with subsequent filling and then delayed emptying into the bowel[49].
CT of abdominal cavity
CT is useful in showing continuity of the cyst with the biliary tree, its relation to surrounding structures and the presence and staging of associated malignancy. It is better than USG in visualization of the intrahepatic bile ducts, distal bile duct and pancreatic head. In patients with type-IVA cysts and Caroli’s disease, it is useful to describe the intrahepatic dilations and the extent of disease (diffuse hepatic or localized segmental involvement). It is important for a surgeon before operation, because localized type-IVA BC or Caroli’s disease can be treated with segmental lobectomy[49]. Computed tomography cholangiography (CTC) after infusion of meglumine iodoxamate with subsequent 3-dimensional rendering has been described in the literature. Fumino et al[60] compared CTC cholangiography with MRCP in visualizing the pancreaticobiliary maljunction in 53 children. They noted superior visualization of the intrahepatic duct and the pancreatic system by MRCP compared with CTC. But the great advantage of CTC was its ability to produce high-quality images without respiratory artifacts in young infants in whom performing a good-quality MRCP is very difficult.
MANAGEMENT IN BC
BC require surgical intervention in order to avoid complications associated with pancreaticobiliary reflux. Management depends on the BC type. Currently, complete cyst excision with cholecystectomy followed by biliary reconstruction using a Roux-en-Y hepatico-jejunostomy is the treatment of choice[53,61,62].
Internal drainage (cystenterostomy) of BC
Currently, this method of treatment has been abandoned due to a high risk of morbidity and malignancy up to 50% following internal biliary drainage. This surgical procedure involved incision of BC and anastomosis it to the duodenum or jejunum depending to its location. Despite relief of clinical symptoms in operated patients, it was associated with a high postoperative mortality rate due to biliary reflux. Reflux of the enteric juice into the retained cyst and biliary tract leaded to recurrent ascending cholangitis. The another complication of this procedure was biliary anastomosis stricture. The most important complication of this treatment method was malignant transformation within the wall of retained BC[62-64]. Therefore, internal drainage is currently not considered to perform and all patients previously operated in this way should be reoperated in order to totally remove the cyst[62,63,65,66].
Total excision of BC with hepaticoenterostomy
Currently, total BC excision is recommended in order to avoid above mentioned complications. Total BC excision with hepaticoenterostomy separates the biliary tree from the pancreatic duct that ends mixing pancreatic juice with bile. In situations where the intensity of fibrosis precludes safe periductal or pericystic dissection, Lilly’s technique is useful. In this technique, the most densely adherent portion of the cyst wall is retained on the hepatoduodenal ligament, removing only the less adherent portion. The mucosal lining of the retained cyst wall should be ablated by diathermy or be stripped or destroyed by abrasion and iodine or alcohol application, because 57% of the bile duct cancer in BC arises from the posterior wall of the cyst[54,63-70].
There are two possible methods of hepaticoenterostomy: hepaticoduodenostomy and Roux-Y hepaticojejunostomy. According to the literature, the success rate of Roux-Y hepaticojejunostomy is 92%, with complication rate of 7%, compared with a complication rate of 42% following hepaticoduodenostomy. Some authors have compared these two reconstructions[63]. Shimotakahara et al[71] did not recommend hepaticoduodenostomy for reconstruction after BC excision due to a higher number of complications (33.3%) such as bilious gastritis due to duodenogastric bile reflux and adhesive bowel obstruction and cholangitis. Contrary to this report, Mukhopadhyay et al[72] recommended hepaticoduodenostomy as a simple and quick procedure with preservation of normal anatomy and physiology and minimum complications.
Some technical aspects of Roux-Y hepaticojejunostomy should be discussed. According to the literature, the anastomosis should be wide in order to prevent the postoperative stricture and Roux-Y jejunal loop should be 40-50 cm long[66]. Conventionally, an adult’s standard of a 40-cm loop is adopted in Roux-Y hepatojejunostomy) also in children, irrespective of patient size. Diao et al[73] compared two patients groups according to Roux-loop length: (1) conventional group (n = 108) where a standard 35-40 cm Roux-loop length was used regardless of the child’s size, and (2) short Roux-loop group (n = 110) in which the Roux-loop length was based on the distance between hepatic hilum and umbilicus. The mean Roux-loop length in patients in the second group was significantly shorter than in the conventional group. There was no significant difference between both groups in age, operative blood loss, operative time, postoperative hospital stay, and duration of drainage. In the conventional group, 2 of (1.8%) 108 patients developed Roux-loop obstruction was observed in 1.8% patients in 1st group and did not appear in 2nd group. Mild reflux was reported in 2 patients in 1st group and 1 patient of 2nd group. Authors concluded that shorter Roux-Y loop according to patient’s size can be safe and effective alternative for biliary reconstruction in children with BC.
Another technical aspect of BC surgery is management with the distal bile duct stump, after cyst excision, which is usually ligated. Diao et al[74] compared two patients groups in order to investigate the feasibility of selectively leaving distal stump unligated. In this study, patients were divided into two groups: (1) non-ligation group (n = 207), where the distal stump was stenotic and was left unligated, and (2) ligation group (n = 63), where the distal stump was not stenotic and was ligated. The pancreatic juice leakage rates were compared. Authors concluded that not ligating distal stump is a feasible approach in patients with the stenotic distal common bile duct. In authors’ opinion, this management simplifies the operative procedure and may minimize pancreatic duct injury.
Different treatment depending on BC type
Total cyst excision with Roux-Y hepatojejunostomy has been recommended by most authors in the treatment of I and IV types of BC[62]. The risk of malignancy in type II and III BC is low. Therefore, complete cyst excision is not necessary. Simple excision of type II BC is sufficient. Choledochoceles often just require endoscopic sphincterotomy in order to allow free duodenal drainage of bile and stones. Large choledochoceles should be treated surgically and excised via duodenostomy because of biliary, duodenal or gastric outlet obstruction. In some cases of small cysts, endoscopic excision is possible. Type IVA BC is treated by surgical cyst excision and a wide hilar hepaticoenterostomy, but clinical symptoms are frequently observed in patients following operation that is caused by the intrahepatic involvement of disease. If the intrahepatic involvement is localized, a segmental hepatectomy can be performed. In case of diffuse disease, a percutaneous hepaticojejunostomy, surgical or endoscopic unroofing of some intrahepatic cysts can be made. Unilobar Caroli’s disease can be treated by hepatic lobectomy. Diffuse Caroli’s disease with recurrent cholangitis, liver failure and cirrhosis and portal hypertension or malignant disease requires orthotopic liver transplantation[61]. There are a lot reports regarding laparoscopic excision of BC with extracorporeal biliary reconstruction. Total intracorporeal biliary reconstruction has been also described in the literature. This minimally invasive treatment method is safe and effective, and it is associated with earlier postoperative feeding and discharge from hospital[75,76].
The timing of BC surgery
Early surgical treatment in order to prevent further complications is recommended. It has been observed that early diagnosis and surgical treatment was associated with a lower morbidity compared with later diagnosis and treatment. Therefore, prenatal diagnosis is such important[77,78]. Foo et al[77] evaluated the clinical outcomes of patients with prenatally diagnosed choledochal cysts compared with those diagnosed after birth and the optimal timing of definitive treatment. The mean age at operation for the prenatally diagnosed group was 4.4 mo. For the postnatal diagnosed group, the mean age at operation was 5.7 years. Based on analysis of 45 patients, they concluded that prenatal diagnosis of BC resulted in earlier definitive surgery. In patients operated in older age, more adverse complications were observed[77]. Tsai et al[79] compared results of surgical treatment according to patients age at the time of surgery. They compared three patients groups that had been operated: < 1 year old (group I, n = 26), 1-16 years old (group II, n = 48), and > 16 years old (group III, n = 33). Group I suffered significantly fewer surgical complications and less severe liver fibrosis compared with groups II and III. Therefore, authors recommended early surgical treatment. Germani et al[80] recommended the surgical treatment after 3 mo of life to increase the success according to the size of the anatomic structure. They recommended the early treatment before 3 mo of life, only in case of severe symptoms.
RESULTS OF THE SURGICAL TREATMENT OF BC
Short-term results and early complications
Surgical treatment of BC is successful in more than 90%. It is associated with a low postoperative morbidity (2.5%-27%) and mortality (0%-6%) rate according to the surgical center and patient’s age. The following early complications have been reported in the literature: biliary-enteric anastomosis dehiscence with biliary fistula, intra-abdominal bile collection (biloma) or abscess and acute peritonitis, pancreatic leakage and pancreatic fistula due to injury to the pancreatic duct, acute pancreatitis, acute cholangitis, ileus caused by bowel obstruction due to intussusception or bowel kinking due to manipulation or adhesions, wound infection and wound dehiscence, gastrointestinal bleeding, hepatic failure, and multiple system organ failure[49,54,64,65,67,68,78,81-87].
Long-term results
Late postoperative complications occur in up to 25% patients and they are the following: biliary-enteric anastomosis stricture, peptic ulcer disease, cholangitis, biliary and intrahepatic stones, pancreatitis, liver failure and biliary cancer. Authors recommend to perform a wide bilio-enteric anastomosis in order to prevent its stricture[49,54,64,67,68,77,80-88]. According to the literature, 0%-6% incidence of malignancy occurs following surgery. It is caused by remnant cyst tissue or subclinical malignant disease which had not been detected before surgery. Therefore, some authors recommend intraoperative endoscopic ultrasonography and histopathological investigation of frozen sections to rule out dysplasia, hyperplasia and malignant disease. All patients with BC require long-term follow-up for bile duct cancer, using ultrasonography and laboratory investigations including liver parameters and cancer markers [carcinoembryonic antigen (CEA), CA 19-9, CA-125][49,87-89]. CA 19-9 is the mosts significant, because it is elevated in up to 85% of patients with cholangiocarcinoma. CEA is raised in about 30% of patients and CA-125 in 40%-50% of patients with cholangiocarcinoma. These markers are not specific for bile duct cancer, because their levels are also raised in other neoplasms and inflammatory diseases[90]. Lee et al[91] analyzed factors predicting cholangiocarcinoma in patients operated for BC. They pointed the following factors: age > 40 years, the absence of a gallstone, elevated CEA or cancer antigen 19-9 serum level, and the presence of anomalous pancreaticobiliary ductal union in univariate analysis, and an elevated cancer antigen 19-9 level in multivariate analysis. Therefore, laboratory investigations of CEA and CA 19-9 in patients following surgery for BC are useful.
Cytology of bile and bile ducts specimens taken during ERCP ora PTC by brush or needle biopsy play an additional role in cholangiocarcinoma diagnosis. Negative cytology from brushing does not exclude malignancy. Combined brush and biopsy cytology specimens increase sensitivity to 40%-70%[90].
Frequency of follow-up in patients following BC resection has not been clearly established in the literature. In my opinion, laboratory investigations including liver parameters and cancer markers (CA 19-9 and CEA) and ultrasonography should be performed as a screening every 6-12 mo.
In cases of the intrahepatic lithiasis and Caroli’s disease, the use of ursodeoxycholic acid (UDCA) in the treatment has been described in the literature. Ros et al[92] UDCA observed that therapy with UDCA caused clinical remission, return to normal liver function, and dissolution of intrahepatic stones on USG in all patients. Therefore, litholytic therapy was indicated by authors for intrahepatic stones in Caroli’s syndrome. Successful treatment of patients with the hepatilithiasis and Caroli’s disease was also described by Guma et al[93].
In conclusion, clinical presentation and management of BC depend on the cyst type according to Todani classification. The early and proper treatment allows to avoid serious complications (including bile duct cancer) in patients with BC. The early treatment is also associated with a lower number of complications. In most cases, total cyst excision with Roux-Y hepaticojejunostomy is the treatment of choice. Surgical treatment of BC is associated with low morbidity and mortality. Patients following surgery for BC require permanent and careful postoperative observation because of possibility of biliary anastomosis stricture and biliary cancer in tissue remnant.
Footnotes
Peer reviewer: Tamir Miloh, MD, Associate Professor, Director of Pediatric Liver/Liver Transplantation Program, Division of Gastroenterology, Phoenix Children’s Hospital, 1919 E Thomas Rd, Main Building, Second Floor, Phoenix, AZ 85016, United States
S- Editor Lv S L- Editor A E- Editor Zhang DN
References
1.
Singham J, Schaeffer D, Yoshida E, Scudamore C. Choledochal cysts: analysis of disease pattern and optimal treatment in adult and paediatric patients. HPB (Oxford). 2007;9:383-387.[PubMed] [DOI]
2.
Singham J, Yoshida EM, Scudamore CH. Choledochal cysts: part 1 of 3: classification and pathogenesis. Can J Surg. 2009;52:434-440.[PubMed]
3.
Jan YY, Chen HM, Chen MF. Malignancy in choledochal cysts. Hepatogastroenterology. 2000;47:337-340.[PubMed]
4.
Bloustein PA. Association of carcinoma with congenital cystic conditions of the liver and bile ducts. Am J Gastroenterol. 1977;67:40-46.[PubMed]
5.
Tan SS, Tan NC, Ibrahim S, Tay KH. Management of adult choledochal cyst. Singapore Med J. 2007;48:524-527.[PubMed] [DOI]
6.
Clifton MS, Goldstein RB, Slavotinek A, Norton ME, Lee H, Farrell J, Nobuhara KK. Prenatal diagnosis of familial type I choledochal cyst. Pediatrics. 2006;117:e596-e600.[PubMed] [DOI]
7.
Søreide K, Søreide JA. Bile duct cyst as precursor to biliary tract cancer. Ann Surg Oncol. 2007;14:1200-1211.[PubMed] [DOI]
8.
Benjamin IS. Biliary cystic disease: the risk of cancer. J Hepatobiliary Pancreat Surg. 2003;10:335-339.[PubMed] [DOI]
9.
Nicholl M, Pitt HA, Wolf P, Cooney J, Kalayoglu M, Shilyansky J, Rikkers LF. Choledochal cysts in western adults: complexities compared to children. J Gastrointest Surg. 2004;8:245-252.[PubMed] [DOI]
10.
Ono S, Sakai K, Kimura O, Iwai N. Development of bile duct cancer in a 26-year-old man after resection of infantile choledochal cyst. J Pediatr Surg. 2008;43:E17-E19.[PubMed] [DOI]
11.
Fujishiro J, Urita Y, Shinkai T, Gotoh C, Hoshino N, Ono K, Komuro H. Clinical characteristics of liver fibrosis in patients with choledochal cysts. J Pediatr Surg. 2011;46:2296-2300.[PubMed] [DOI]
12.
Saluja SS, Nayeem M, Sharma BC, Bora G, Mishra PK. Management of choledochal cysts and their complications. Am Surg. 2012;78:284-290.[PubMed]
13.
Tongprasert F, Traisrisilp K, Tongsong T. Prenatal diagnosis of choledochal cyst: a case report. J Clin Ultrasound. 2012;40:48-50.[PubMed] [DOI]
14.
Tanaka N, Ueno T, Takama Y, Fukuzawa M. Diagnosis and management of biliary cystic malformations in neonates. J Pediatr Surg. 2010;45:2119-2123.[PubMed] [DOI]
15.
Chiang L, Chui CH, Low Y, Jacobsen AS. Perforation: a rare complication of choledochal cysts in children. Pediatr Surg Int. 2011;27:823-827.[PubMed] [DOI]
16.
Gong L, Qu Q, Xiang X, Wang J. Clinical analysis of 221 cases of adult choledochal cysts. Am Surg. 2012;78:414-418.[PubMed]
17.
Okada T, Itoh T, Sasaki F, Cho K, Honda S, Todo S. Comparison between prenatally diagnosed choledochal cyst and type-1 cystic biliary atresia by CD56-immunostaining using liver biopsy specimens. Eur J Pediatr Surg. 2007;17:6-11.[PubMed] [DOI]
18.
Muise AM, Turner D, Wine E, Kim P, Marcon M, Ling SC. Biliary atresia with choledochal cyst: implications for classification. Clin Gastroenterol Hepatol. 2006;4:1411-1414.[PubMed] [DOI]
19.
Kouraklis G, Misiakos E, Glinavou A, Karatzas G, Gogas J, Skalkeas G. Cystic dilatations of the common bile duct in adults. HPB Surg. 1996;10:91-94; discussion 94-95.[PubMed] [DOI]
20.
Scudamore CH, Hemming AW, Teare JP, Fache JS, Erb SR, Watkinson AF. Surgical management of choledochal cysts. Am J Surg. 1994;167:497-500.[PubMed] [DOI]
21.
Lopez RR, Pinson CW, Campbell JR, Harrison M, Katon RM. Variation in management based on type of choledochal cyst. Am J Surg. 1991;161:612-615.[PubMed] [DOI]
22.
Katyal D, Lees GM. Choledochal cysts: a retrospective review of 28 patients and a review of the literature. Can J Surg. 1992;35:584-588.[PubMed]
23.
Yotsuyanagi S. Contributions to etiology and pathogeny of idiopathic cystic dilatation of the common bile duct with report of three cases: new etiological theory based on supposed inequal epithelial proliferation at the stage of physiological epithelial occlusion of primitive choledochus. Gann. 1936;30:601-650.
24.
Babbitt DP. [Congenital choledochal cysts: new etiological concept based on anomalous relationships of the common bile duct and pancreatic bulb]. Ann Radiol (Paris). 1969;12:231-240.[PubMed]
25.
Sugiyama M, Haradome H, Takahara T, Izumisato Y, Abe N, Masaki T, Mori T, Hachiya J, Atomi Y. Biliopancreatic reflux via anomalous pancreaticobiliary junction. Surgery. 2004;135:457-459.[PubMed] [DOI]
26.
Todani T, Narusue M, Watanabe Y, Tabuchi K, Okajima K. Management of congenital choledochal cyst with intrahepatic involvement. Ann Surg. 1978;187:272-280.[PubMed] [DOI]
27.
Jeong IH, Jung YS, Kim H, Kim BW, Kim JW, Hong J, Wang HJ, Kim MW, Yoo BM, Kim JH. Amylase level in extrahepatic bile duct in adult patients with choledochal cyst plus anomalous pancreatico-biliary ductal union. World J Gastroenterol. 2005;11:1965-1970.[PubMed]
28.
Zhan JH, Hu XL, Dai CJ, Niu J, Gu JQ. Expressions of p53 and inducible nitric oxide synthase in congenital choledochal cysts. Hepatobiliary Pancreat Dis Int. 2004;3:120-123.[PubMed]
29.
Iwai N, Yanagihara J, Tokiwa K, Shimotake T, Nakamura K. Congenital choledochal dilatation with emphasis on pathophysiology of the biliary tract. Ann Surg. 1992;215:27-30.[PubMed] [DOI]
30.
Imazu M, Iwai N, Tokiwa K, Shimotake T, Kimura O, Ono S. Factors of biliary carcinogenesis in choledochal cysts. Eur J Pediatr Surg. 2001;11:24-27.[PubMed] [DOI]
31.
Hosoki T, Hasuike Y, Takeda Y, Michita T, Watanabe Y, Sakamori R, Tokuda Y, Yutani K, Sai C, Mitomo M. Visualization of pancreaticobiliary reflux in anomalous pancreaticobiliary junction by secretin-stimulated dynamic magnetic resonance cholangiopancreatography. Acta Radiol. 2004;45:375-382.[PubMed] [DOI]
32.
Okada A, Hasegawa T, Oguchi Y, Nakamura T. Recent advances in pathophysiology and surgical treatment of congenital dilatation of the bile duct. J Hepatobiliary Pancreat Surg. 2002;9:342-351.[PubMed] [DOI]
33.
Komi N, Takehara H, Kunitomo K, Miyoshi Y, Yagi T. Does the type of anomalous arrangement of pancreaticobiliary ducts influence the surgery and prognosis of choledochal cyst?. J Pediatr Surg. 1992;27:728-731.[PubMed] [DOI]
34.
Kim OH, Chung HJ, Choi BG. Imaging of the choledochal cyst. Radiographics. 1995;15:69-88.[PubMed]
35.
Cheng SP, Yang TL, Jeng KS, Liu CL, Lee JJ, Liu TP. Choledochal cyst in adults: aetiological considerations to intrahepatic involvement. ANZ J Surg. 2004;74:964-967.[PubMed] [DOI]
36.
Davenport M, Basu R. Under pressure: choledochal malformation manometry. J Pediatr Surg. 2005;40:331-335.[PubMed] [DOI]
37.
Alonso-LEJ F, Rever WB, Pessagno DJ. Congenital choledochal cyst, with a report of 2, and an analysis of 94, cases. Int Abstr Surg. 1959;108:1-30.[PubMed]
38.
Todani T, Watanabe Y, Narusue M, Tabuchi K, Okajima K. Congenital bile duct cysts: Classification, operative procedures, and review of thirty-seven cases including cancer arising from choledochal cyst. Am J Surg. 1977;134:263-269.[PubMed]
39.
Todani T, Watanabe Y, Toki A, Morotomi Y. Classification of congenital biliary cystic disease: special reference to type Ic and IVA cysts with primary ductal stricture. J Hepatobiliary Pancreat Surg. 2003;10:340-344.[PubMed] [DOI]
40.
Michaelides M, Dimarelos V, Kostantinou D, Bintoudi A, Tzikos F, Kyriakou V, Rodokalakis G, Tsitouridis I. A new variant of Todani type I choledochal cyst. Imaging evaluation. Hippokratia. 2011;15:174-177.[PubMed]
41.
Ziegler KM, Pitt HA, Zyromski NJ, Chauhan A, Sherman S, Moffatt D, Lehman GA, Lillemoe KD, Rescorla FJ, West KW. Choledochoceles: are they choledochal cysts?. Ann Surg. 2010;252:683-690.[PubMed]
42.
Ziegler KM, Zyromski NJ. Choledochoceles: are they choledochal cysts?. Adv Surg. 2011;45:211-224.[PubMed] [DOI]
43.
Lendoire J, Barros Schelotto P, Alvarez Rodríguez J, Duek F, Quarin C, Garay V, Amante M, Cassini E, Imventarza O. Bile duct cyst type V (Caroli's disease): surgical strategy and results. HPB (Oxford). 2007;9:281-284.[PubMed] [DOI]
44.
Caroli J, Soupault R, Kossakowski J, Plocker L. [Congenital polycystic dilation of the intrahepatic bile ducts; attempt at classification]. Sem Hop. 1958;34:488-95/SP.[PubMed]
45.
Caroli J, Couinaud C, Soupault R, Porcher P, Eteve J. [A new disease, undoubtedly congenital, of the bile ducts: unilobar cystic dilation of the hepatic ducts]. Sem Hop. 1958;34:496-502/SP.[PubMed]
46.
Shedda S, Robertson A. Caroli's syndrome and adult polycystic kidney disease. ANZ J Surg. 2007;77:292-294.[PubMed] [DOI]
47.
Kim JT, Hur YJ, Park JM, Kim MJ, Park YN, Lee JS. Caroli's syndrome with autosomal recessive polycystic kidney disease in a two month old infant. Yonsei Med J. 2006;47:131-134.[PubMed] [DOI]
48.
Visser BC, Suh I, Way LW, Kang SM. Congenital choledochal cysts in adults. Arch Surg. 2004;139:855-60; discussion 860-2.[PubMed] [DOI]
49.
Singham J, Yoshida EM, Scudamore CH. Choledochal cysts: part 2 of 3: Diagnosis. Can J Surg. 2009;52:506-511.[PubMed]
50.
Atkinson HD, Fischer CP, de Jong CH, Madhavan KK, Parks RW, Garden OJ. Choledochal cysts in adults and their complications. HPB (Oxford). 2003;5:105-110.[PubMed]
51.
Kiresi DA, Karabacakoğlu A, Dilsiz A, Karaköse S. Spontaneous rupture of choledochal cyst presenting in childhood. Turk J Pediatr. 2005;47:283-286.[PubMed]
52.
Todani T, Tabuchi K, Watanabe Y, Kobayashi T. Carcinoma arising in the wall of congenital bile duct cysts. Cancer. 1979;44:1134-1141.[PubMed]
53.
Lipsett PA, Pitt HA, Colombani PM, Boitnott JK, Cameron JL. Choledochal cyst disease. A changing pattern of presentation. Ann Surg. 1994;220:644-652.[PubMed] [DOI]
54.
Jesudason SR, Jesudason MR, Mukha RP, Vyas FL, Govil S, Muthusami JC. Management of adult choledochal cysts--a 15-year experience. HPB (Oxford). 2006;8:299-305.[PubMed]
55.
Irie H, Honda H, Jimi M, Yokohata K, Chijiiwa K, Kuroiwa T, Hanada K, Yoshimitsu K, Tajima T, Matsuo S. Value of MR cholangiopancreatography in evaluating choledochal cysts. AJR Am J Roentgenol. 1998;171:1381-1385.[PubMed]
56.
Lee HK, Park SJ, Yi BH, Lee AL, Moon JH, Chang YW. Imaging features of adult choledochal cysts: a pictorial review. Korean J Radiol. 2009;10:71-80.[PubMed] [DOI]
57.
Chen CP. Ultrasound-guided needle aspiration of a fetal choledochal cyst. Ultrasound Obstet Gynecol. 2001;17:175-176.[PubMed]
58.
Bianchi DW, Crombleholme TM, D’Alton ME. Choledochal cyst. In:Bianchi DW, Crombleholme TM, D’Alton ME.,editors.Fetology,Diagnosis and Management of the Fetal Patient. New York: McGraw-Hill; 2000.pp.507-514.
59.
Yeom SK, Lee SW, Cha SH, Chung HH, Je BK, Kim BH, Hyun JJ. Biliary reflux detection in anomalous union of the pancreatico-biliary duct patients. World J Gastroenterol. 2012;18:952-959.[PubMed] [DOI]
60.
Fumino S, Ono S, Kimura O, Deguchi E, Iwai N. Diagnostic impact of computed tomography cholangiography and magnetic resonance cholangiopancreatography on pancreaticobiliary maljunction. J Pediatr Surg. 2011;46:1373-1378.[PubMed] [DOI]
61.
Shi LB, Peng SY, Meng XK, Peng CH, Liu YB, Chen XP, Ji ZL, Yang DT, Chen HR. Diagnosis and treatment of congenital choledochal cyst: 20 years' experience in China. World J Gastroenterol. 2001;7:732-734.[PubMed]
62.
Singham J, Yoshida EM, Scudamore CH. Choledochal cysts. Part 3 of 3: management. Can J Surg. 2010;53:51-56.[PubMed]
63.
Watanabe Y, Toki A, Todani T. Bile duct cancer developed after cyst excision for choledochal cyst. J Hepatobiliary Pancreat Surg. 1999;6:207-212.[PubMed] [DOI]
64.
Todani T, Watanabe Y, Toki A, Urushihara N, Sato Y. Reoperation for congenital choledochal cyst. Ann Surg. 1988;207:142-147.[PubMed] [DOI]
65.
Saing H, Han H, Chan KL, Lam W, Chan FL, Cheng W, Tam PK. Early and late results of excision of choledochal cysts. J Pediatr Surg. 1997;32:1563-1566.[PubMed] [DOI]
66.
Tao KS, Lu YG, Wang T, Dou KF. Procedures for congenital choledochal cysts and curative effect analysis in adults. Hepatobiliary Pancreat Dis Int. 2002;1:442-445.[PubMed]
67.
Mabrut JY, Bozio G, Hubert C, Gigot JF. Management of congenital bile duct cysts. Dig Surg. 2010;27:12-18.[PubMed] [DOI]
68.
Kawarada Y, Das BC, Tabata M, Isaji S. Surgical treatment of type IV choledochal cysts. J Hepatobiliary Pancreat Surg. 2009;16:684-687.[PubMed] [DOI]
69.
Lilly JR. The surgical treatment of choledochal cyst. Surg Gynecol Obstet. 1979;149:36-42.[PubMed]
70.
Flanigan DP. Biliary carcinoma associated with biliary cysts. Cancer. 1977;40:880-883.[PubMed]
71.
Shimotakahara A, Yamataka A, Yanai T, Kobayashi H, Okazaki T, Lane GJ, Miyano T. Roux-en-Y hepaticojejunostomy or hepaticoduodenostomy for biliary reconstruction during the surgical treatment of choledochal cyst: which is better?. Pediatr Surg Int. 2005;21:5-7.[PubMed] [DOI]
72.
Mukhopadhyay B, Shukla RM, Mukhopadhyay M, Mandal KC, Mukherjee PP, Roy D, Biswas SK, Basu KS. Choledochal cyst: A review of 79 cases and the role of hepaticodochoduodenostomy. J Indian Assoc Pediatr Surg. 2011;16:54-57.[PubMed] [DOI]
73.
Diao M, Li L, Zhang JZ, Cheng W. A shorter loop in Roux-Y hepatojejunostomy reconstruction for choledochal cysts is equally effective: preliminary results of a prospective randomized study. J Pediatr Surg. 2010;45:845-847.[PubMed] [DOI]
74.
Diao M, Li L, Cheng W. Is it necessary to ligate distal common bile duct stumps after excising choledochal cysts?. Pediatr Surg Int. 2011;27:829-832.[PubMed] [DOI]
75.
Gander JW, Cowles RA, Gross ER, Reichstein AR, Chin A, Zitsman JL, Middlesworth W, Rothenberg SS. Laparoscopic excision of choledochal cysts with total intracorporeal reconstruction. J Laparoendosc Adv Surg Tech A. 2010;20:877-881.[PubMed] [DOI]
76.
Lee H, Hirose S, Bratton B, Farmer D. Initial experience with complex laparoscopic biliary surgery in children: biliary atresia and choledochal cyst. J Pediatr Surg. 2004;39:804-807.[PubMed] [DOI]
77.
Foo DC, Wong KK, Lan LC, Tam PK. Impact of prenatal diagnosis on choledochal cysts and the benefits of early excision. J Paediatr Child Health. 2009;45:28-30.[PubMed] [DOI]
78.
She WH, Chung HY, Lan LC, Wong KK, Saing H, Tam PK. Management of choledochal cyst: 30 years of experience and results in a single center. J Pediatr Surg. 2009;44:2307-2311.[PubMed] [DOI]
79.
Tsai MS, Lin WH, Hsu WM, Lai HS, Lee PH, Chen WJ. Clinicopathological feature and surgical outcome of choledochal cyst in different age groups: the implication of surgical timing. J Gastrointest Surg. 2008;12:2191-2195.[PubMed] [DOI]
80.
Germani M, Liberto D, Elmo G, Lobos P, Ruiz E. Choledochal cyst in pediatric patients: a 10 years single institution experience. Acta Gastroenterol Latinoam. 2011;41:302-307.[PubMed]
81.
Cho MJ, Hwang S, Lee YJ, Kim KH, Ahn CS, Moon DB, Lee SK, Kim MH, Lee SS, Park DH. Surgical experience of 204 cases of adult choledochal cyst disease over 14 years. World J Surg. 2011;35:1094-1102.[PubMed] [DOI]
82.
Takeshita N, Ota T, Yamamoto M. Forty-year experience with flow-diversion surgery for patients with congenital choledochal cysts with pancreaticobiliary maljunction at a single institution. Ann Surg. 2011;254:1050-1053.[PubMed] [DOI]
83.
Zheng LX, Jia HB, Wu DQ, Shang H, Zhong XY, Wang QS, Zhou WX, Sun ZH. Experience of congenital choledochal cyst in adults: treatment, surgical procedures and clinical outcome in the Second Affiliated Hospital of Harbin Medical University. J Korean Med Sci. 2004;19:842-847.[PubMed] [DOI]
84.
Jordan PH, Goss JA, Rosenberg WR, Woods KL. Some considerations for management of choledochal cysts. Am J Surg. 2004;187:434-439.[PubMed] [DOI]
85.
Lee SC, Kim HY, Jung SE, Park KW, Kim WK. Is excision of a choledochal cyst in the neonatal period necessary?. J Pediatr Surg. 2006;41:1984-1986.[PubMed] [DOI]
86.
Li MJ, Feng JX, Jin QF. Early complications after excision with hepaticoenterostomy for infants and children with choledochal cysts. Hepatobiliary Pancreat Dis Int. 2002;1:281-284.[PubMed]
87.
Kim JW, Moon SH, Park do H, Lee SS, Seo DW, Kim MH, Lee SK. Course of choledochal cysts according to the type of treatment. Scand J Gastroenterol. 2010;45:739-745.[PubMed] [DOI]
88.
Kobayashi S, Asano T, Yamasaki M, Kenmochi T, Nakagohri T, Ochiai T. Risk of bile duct carcinogenesis after excision of extrahepatic bile ducts in pancreaticobiliary maljunction. Surgery. 1999;126:939-944.[PubMed] [DOI]
89.
Franko J, Nussbaum ML, Morris JB. Choledochal cyst cholangiocarcinoma arising from adenoma: case report and a review of the literature. Curr Surg. 2006;63:281-284.[PubMed] [DOI]
90.
Khan SA, Davidson BR, Goldin R, Pereira SP, Rosenberg WM, Taylor-Robinson SD, Thillainayagam AV, Thomas HC, Thursz MR, Wasan H. Guidelines for the diagnosis and treatment of cholangiocarcinoma: consensus document. Gut. 2002;51 Suppl 6:VI1-VI9.[PubMed] [DOI]
91.
Lee SE, Jang JY, Lee YJ, Choi DW, Lee WJ, Cho BH, Kim SW. Choledochal cyst and associated malignant tumors in adults: a multicenter survey in South Korea. Arch Surg. 2011;146:1178-1184.[PubMed] [DOI]
92.
Ros E, Navarro S, Bru C, Gilabert R, Bianchi L, Bruguera M. Ursodeoxycholic acid treatment of primary hepatolithiasis in Caroli's syndrome. Lancet. 1993;342:404-406.[PubMed] [DOI]
93.
Guma C, Viola C, Apestegui M, Thomé U, Tani D, Kido N, Yoshida R, Coconi J, Wulfson A, Findor A. [Hepatolithiasis and Caroli's disease in Argentina: results of a multicenter study]. Acta Gastroenterol Latinoam. 1999;29:9-15.[PubMed]