World J Gastroenterol. 2012 August 28; 18(32): 4399-4403.
Published online 2012 August 28. doi: 10.3748/wjg.v18.i32.4399.
Tissue transglutaminase levels above 100 U/mL and celiac disease: A prospective study
Amani Mubarak, Victorien M Wolters, Frits HJ Gmelig-Meyling, Fiebo JW ten Kate and Roderick HJ Houwen.
Amani Mubarak, Victorien M Wolters, Roderick HJ Houwen, Department of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Frits HJ Gmelig-Meyling, Department of Immunology, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Fiebo JW ten Kate, Department of Pathology, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands
Author contributions: Mubarak A coordinated the study, selected patients for inclusion, performed the data-analysis and wrote the article; Wolters VM selected patients for inclusion and reviewed the article; Gmelig-Meyling FHJ supervised the performance of serological testing and reviewed the article; ten Kate FJW classified all biopsies and reviewed the article; and Houwen RHJ supervised the whole project and reviewed the article.
Correspondence to: Dr. Amani Mubarak, Department of Pediatric Gastroenterology, Wilhelmina Children’s hospital, University Medical Center Utrecht, KE 01.144.3, PO Box 85090, 3508 AB Utrecht, The Netherlands. a.mubarak@umcutrecht.nl
Telephone: +31-88-7555294 Fax: +31-88-7555348
Received June 8, 2012; Revised July 24, 2012; Accepted July 28, 2012;
Abstract
AIM: To investigate whether a tissue-transglutaminase antibody (tTGA) level ≥ 100 U/mL is sufficient for the diagnosis of celiac disease (CD).
METHODS: Children suspected of having CD were prospectively included in our study between March 2009 and September 2011. All patients with immune globulin A deficiency and all patients on a gluten-free diet were excluded from the study. Anti-endomysium antibodies (EMA) were detected by means of immunofluorescence using sections of distal monkey esophagus (EUROIMMUN, Luebeck, Germany). Serum anti-tTGA were measured by means of enzyme-linked immunosorbent assay using human recombinant tissue transglutaminase (ELiA Celikey IgA kit Phadia AB, Uppsala, Sweden). The histological slides were graded by a single experienced pathologist using the Marsh classification as modified by Oberhuber. Marsh II and III lesions were considered to be diagnostic for the disease. The positive predictive values (PPVs), negative predictive values (NPVs), sensitivity and specificity of EMA and tTGA along with their 95% CI (for the cut off values > 10 and ≥ 100 U/mL) were calculated using histology as the gold standard for CD.
RESULTS: A total of 183 children were included in the study. A total of 70 (38.3%) were male, while 113 (61.7%) were female. The age range was between 1.0 and 17.6 years, and the mean age was 6.2 years. One hundred twenty (65.6%) patients had a small intestinal biopsy diagnostic for the disease; 3 patients had a Marsh II lesion, and 117 patients had a Marsh III lesion. Of the patients without CD, only 4 patients had a Marsh I lesion. Of the 183 patients, 136 patients were positive for EMA, of whom 20 did not have CD, yielding a PPV for EMA of 85% (95% CI: 78%-90%) and a corresponding specificity of 68% (95% CI: 55%-79%). The NPV and specificity for EMA were 91% (95% CI: 79%-97%) and 97% (95% CI: 91%-99%), respectively. Increased levels of tTGA were found in 130 patients, although only 116 patients truly had histological evidence of the disease. The PPV for tTGA was 89% (95% CI: 82%-94%), and the corresponding specificity was 78% (95% CI: 65%-87%). The NPV and sensitivity were 92% (95% CI: 81%-98%) and 97% (95% CI: 91%-99%), respectively. A tTGA level ≥ 100 U/mL was found in 87 (47.5%) patients, all of whom were also positive for EMA. In all these 87 patients, epithelial lesions confirming CD were found, giving a PPV of 100% (95%CI: 95%-100%). The corresponding specificity for this cut-off value was also 100% (95% CI: 93%-100%). Within this group, a total of 83 patients had symptoms, at least gastrointestinal and/or growth retardation. Three patients were asymptomatic but were screened because they belonged to a group at risk for CD (diabetes mellitus type 1 or positive family history). The fourth patient who lacked CD-symptoms was detected by coincidence during an endoscopy performed for gastro-intestinal bleeding.
CONCLUSION: This study confirms based on prospective data that a small intestinal biopsy is not necessary for the diagnosis of CD in symptomatic patients with tTGA ≥ 100 U/mL.
Keywords: Celiac disease, Diagnosis, Serology, Anti-tissue-transglutaminase antibodies, Anti-endomysium antibodies
INTRODUCTION
Celiac disease (CD) is an immune-mediated enteropathy affecting approximately 1% of the worldwide population[1-3]. The immune reaction occurs when genetically susceptible individuals ingest gluten, which is a storage protein in wheat and the related grain species barley and rye, and this reaction is completely reversible upon gluten withdrawal, which is currently the only available treatment for CD[3-5]. The gold standard for the diagnosis of CD has been considered to be a small intestinal biopsy since the histological lesions of CD were discovered in 1954[6,7]. However, a small intestinal biopsy is not only expensive, time-consuming and stressful for children and their parents but may also provide inconclusive or even false results, due to patchy disease or to inadequate quality or orientation of the biopsy specimen[8-10]. Therefore, there has long been research focused on finding non-invasive markers to diagnose CD. For this purpose, the disease-associated auto-antibodies, especially anti-endomysium antibodies (EMA) and anti-tissue-transglutaminase antibodies (tTGA), have proven to be highly sensitive and specific[6,11-14]. In fact, according to the new ESPGHAN guidelines for the diagnosis of CD, a confirmatory small intestinal biopsy is no longer necessary in genetically predisposed individuals who are symptomatic and who have a tTGA of at least 10 times the upper limit of normal, a positive EMA and a good clinical response to the gluten free diet[15]. However, these new guidelines for children are mainly based on retrospective data[16-18]. Because such study designs are subject to selection bias, and because the diagnosis of CD implies a lifelong gluten free diet, the diagnosis of CD should be based on serology only when the chance of a false positive result is close to zero. The aim of the present study was therefore to evaluate prospectively whether the new diagnostic approach in patients with high tTGA levels is justified.
MATERIALS AND METHODS
Study population
All patients who were referred to the Wilhelmina Children’s Hospital in Utrecht, the Netherlands with the suspicion of having CD were prospectively included in the study between March 2009 and September 2011. Patients were referred to us because of symptoms associated with CD (e.g., abdominal symptoms, growth retardation) or because they belonged to a group at risk for CD, e.g., patients with Down syndrome or Diabetes Mellitus and patients with a positive family history for CD. In this patient group, serology (both EMA and tTGA) was performed, and any patient with abnormal serology was biopsied, as were patients with negative serology but a high clinical suspicion of CD. Patients with immunoglobulin A (IgA) deficiency (n = 8) and patients on gluten restriction during the diagnostic work-up were excluded from the study. The study was performed according to the guidelines of the local medical ethics board.
Serological assessment
IgA EMA values were detected by indirect immunofluorescence using sections of distal monkey esophagus mounted on glass slides (EUROIMMUN, Luebeck, Germany). Serum IgA tTGA values were measured using the ELiA Celikey IgA kit (Phadia AB, Uppsala, Sweden). As recommended by the manufacturer, serum samples containing an antibody titer greater than 10 U/mL were considered positive. Total IgA was measured in all patients, and a serum IgA concentration below 0.07 g/L was regarded as IgA deficiency.
Histological evaluation
Duodenal biopsies were obtained by upper gastrointestinal endoscopy. An average of 3.1 biopsies (range: 1-8 biopsies) per patient were taken from the distal duodenum. Starting at the end of 2009, duodenal bulb biopsies were also routinely obtained during endoscopy, as recent studies suggested that this region could be the only affected site in CD[19]. On average, 1.9 biopsies per patient were taken from this location with a range of 0 to 5.
Histological diagnosis for all patients was performed by a single experienced pathologist using the Marsh classification as modified by Oberhuber[20,21]. The pathologist had no knowledge of the serological results or of the clinical presentation of the patients. An increased number of intraepithelial lymphocytes (Marsh I) were considered not to be diagnostic for CD. By contrast, Marsh I combined with crypt hyperplasia (i.e., Marsh II) or findings with villous atrophy (Marsh III) were considered to be diagnostic for CD.
Statistical analysis
The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the screening tests, which exhibited 95% confidence intervals (CI), were calculated using the histological evaluation as the gold standard. It was subsequently determined whether a tTGA level ≥ 100 U/mL is associated with a nearly perfect PPV.
RESULTS
A total of 183 patients met the inclusion criteria of the study. Of those patients, 70 (38.3%) were male, and 113 (61.7%) female with an age range of between 1.0 and 17.6 years and a mean age of 6.2 years. A total of 120 (65.6%) patients had a biopsy diagnostic for CD, of whom only 3 patients had a Marsh II lesion. In the remaining 63 (34.4%) patients, the diagnosis of CD could be excluded. Of the patients without CD, only 4 patients had Marsh I histology.
Of the total study population, 138 patients had positive EMA and⁄or tTGA antibodies, while 45 patients were negative for both antibodies. The patients who were negative for both antibodies underwent a small intestinal biopsy because of a strong clinical suspicion of CD (CD-like symptoms). Three of these patients had a Marsh III lesion, and one patient had a Marsh II lesion, while the diagnosis of CD could be excluded in the remaining 41 patients.
A positive EMA was found in 136 (74.3%) patients; 20 (31.7%) of them did not meet the histological criteria for CD (Table 1), giving a specificity of only 68% (Table 2). The corresponding PPV was 85%. The specificity of tTGA was slightly better (78%), with 116 of 130 positive patients being correctly diagnosed (Table 1). The corresponding PPV was also better at 89% (Table 2).
Table 1
Table 1
Results of small-intestinal biopsy and serology n (%)
Table 2
Table 2
Sensitivity, specificity, positive predictive value and negative predictive value of anti-endomysium antibodies and anti-tissue-transglutaminase antibodies (%)
EMA was undetectable in 47 (25.7%) patients, of whom 43 indeed showed normal histology (Table 1). Consequently, the sensitivity and NPV of EMA were high with values of 97% and 91%, respectively (Table 2). These values were equally high for tTGA, i.e., 97% and 92%, respectively. Illustratively, 49 of the 53 patients with negative tTGA did not have CD (Table 1).
A total of 42 patients (23.0%) had tTGA levels between 10 and 100 U/mL. Of those patients, only 28 (66.7%) had CD, while the diagnosis could be histologically excluded in 14 (33.3%) patients. Of the latter group, 3 patients had a Marsh I lesion. By contrast, the 87 patients with a tTGA level ≥ 100 U/mL all met the histological criteria for CD (Table 1), yielding a PPV of 100% (Table 2). All were also positive for EMA. Among these 87 patients, only 4 patients were asymptomatic. Three patients were screened because they belonged to a group at risk for CD (diabetes mellitus type 1 or a positive family history for CD). The fourth patient who lacked CD-symptoms was detected by coincidence during an endoscopy performed for gastro-intestinal bleeding. All other patients (n = 83) had typical symptoms (at least gastro-intestinal symptoms and/or growth retardation). After the diagnosis of CD was made, all patients adhered to the gluten-free diet, and the vast majority showed clinical improvement.
DISCUSSION
In patients with high tTGA levels, there is increasing evidence that a small intestinal biopsy is not needed to confirm the diagnosis of CD, as these increased levels are highly suggestive of the disease. This conclusion was also stated in the new ESPGHAN guidelines for the diagnosis of CD in the pediatric population[15]. Briefly, these guidelines suggest that in symptomatic individuals who have tTGA levels of at least 10 times the upper limit of normal and who respond well to the gluten free diet, histological confirmation is unnecessary. However, prospective studies are needed to confirm the applicability of these guidelines in clinical practice.
The sole reliance on serology for the diagnosis of CD is appropriate only if the PPV is close to 100%. In this study, it was prospectively shown that 87/87 patients with a tTGA of at least 100 U/mL did indeed suffer from CD, giving a PPV of 100%. However, in this cohort, most of the patients had typical CD symptoms and responded well to the diet, while only 4 patients lacked any CD associated symptoms. Therefore, due to the under-representation of asymptomatic patients in this cohort, it can be questioned whether this perfect PPV will also be observed in asymptomatic patients.
Comparable results were found in previous retrospective studies, showing that high tTGA levels are associated with histological lesions compatible with CD[16-18]. Barker et al[22] showed that 48 of 49 mostly symptomatic children with a tTGA level ≥100 U/mL had at least Marsh II enteropathy. Comparably, Donaldson et al[23] showed that 38 of the 38 pediatric patients with tTGA ≥100 U/mL had Marsh III histopathology. A subsequent retrospective study, also in a pediatric population, showed that all symptomatic patients with tTGA of at least 100 U/mL who responded well to the diet had CD (n = 111), thereby reaching a PPV of 100%[24].
Similarly, in a study conducted in a mixed adult/pediatric population, it was shown that a tTGA ≥ 100 U/mL occurs almost exclusively in the setting of Marsh III (73 of 76 patients) and that the 3 patients without villous atrophy had either a Marsh II (n = 2) or a Marsh I (n = 1) lesion[25]. Likewise, a study performed in adults showed that 91 patients with a tTGA level of at least 10 times the upper limit all had at least Marsh II enteropathy[16]. By contrast, Freeman reported that 3 of 14 adult patients with tTGA ≥ 100 U/mL did not have CD[26]. Notably, in the latter 3 studies, an exact description of the clinical presentation of the patients was lacking[16,25,26].
To the best of our knowledge, only one other prospective study has been performed in a mixed pediatric and adult population. This study showed that 1 of the 72 patients with a tTGA of at least 11.4 times the upper limit of normal had a normal small intestinal biopsy, yielding a PPV of 98.6%, which the authors considered to be insufficient for omitting a biopsy[27]. However, in this study, the presence of symptoms was not taken into consideration, which may influence the PPV. In fact, the patient with this high level of tTGA and a normal biopsy did have an excellent clinical and serological response to the diet, suggesting that CD may have been missed histologically.
In conclusion, the current study shows that 87/87 patients with tTGA ≥ 100 U/mL had CD, which confirms the new ESPGHAN guidelines and other retrospective studies. However, because almost all studied patients in this study were symptomatic, omitting a biopsy should only be considered in this group. By contrast, in asymptomatic individuals, a small intestinal biopsy should still be performed, at least until more studies become available studying this specific group.
ACKNOWLEDGMENTS
The authors would like to thank Willy de Kruijf for performing the EMA and tTGA analysis.
COMMENTS
Background
In celiac disease (CD), the ingestion of gluten leads to a typical enteropathy characterized by an increase in the number of intra-epithelial lymphocytes, hyperplasia of the crypts and, in most cases, atrophy of the villi. The detection of these lesions in small intestinal biopsy specimens obtained by upper endoscopy is used to identify CD and is considered to be the gold standard investigation for the diagnosis of the disease. Serologically, the immune reaction to gluten can be detected by measuring disease-specific antibodies, the anti-endomysium antibodies (EMA) and the anti-tissue-transglutaminase antibodies (tTGA). In clinical practice, the measurement of these antibodies is used to screen for CD, as they are highly sensitive and specific. The aim of this study is to determine to what extent serology can replace a small intestinal biopsy for the diagnosis of CD.
Research frontiers
Retrospective studies have shown that a notably high titer of tTGA is strongly associated with the degree of enteropathy. Thus, high levels of tTGA seem to be sufficient for the diagnosis of CD. Therefore, the ESPGHAN guidelines for the diagnosis of CD in children and adolescents were revised this year, recommending that the diagnosis of CD can be made without histological confirmation in genetically predisposed children who are symptomatic, have a tTGA level of at least 10 times the upper limit of normal, as well as a positive EMA, and who respond well to the diet.
Innovations and breakthroughs
Because the new guidelines are based only on retrospective data, which allow a chance of selection bias, there was need for a prospective study to evaluate the guidelines in clinical practice. The authors therefore prospectively investigated the positive predictive value of tTGA ≥ 100 U/mL (= 10 times the upper limit) and demonstrated that it indeed reached 100%; i.e., all patients with these high tTGA values showed the small intestinal lesions that are diagnostic for CD. Therefore, in these patients, histological confirmation seems to be unnecessary. Because the majority of the studied patients had typical CD-symptoms and all of them were positive for EMA, omitting a biopsy should only be considered for symptomatic patients who also have increased levels of EMA.
Applications
The results of this study show that the new ESPGHAN guidelines in patients with very high tTGA levels can be used safely without being at risk for over-diagnosing the disease. Because a significant number of patients fulfill these criteria, i.e., almost half of the patients in this study, applying this guideline will have great clinical implications. Omitting a biopsy will not only reduce the stress and inconvenience associated with the diagnosis, but will also save time and significantly reduce costs.
Terminology
tTGA: These anti-bodies are directed against the enzyme tissue-transglutaminase, which is the auto-antigen in CD. This enzyme plays an essential role in eliciting the immune response against gluten; EMA: The endomysium is the intercellular matrix, which lies between the smooth muscle cells of the muscularis mucosae throughout the gastro-intestinal tract. It is rich in the enzyme tissue-transglutaminase. Antibodies directed against the endomysium are actually directed against tissue-transglutaminase.
Peer review
The authors present data from a prospective study on application of new cut-off values for diagnosing celiac disease in a population of children with high pre-test likelihood of disease. In the presented population, the specificity reaches 100 % if cut-off is raised to 10 times the regular cut-off. The draw-back is loss of specificity, but the subgroup with high titre can be diagnosed without duodenal biopsies. Upper endoscopy is demanding for children and mostly is performed in general anaesthesia, with the implications that may have for complications etc. The manuscript is well written, with a clear aim and conclusion.
Footnotes
Peer reviewers: Rasmus Goll, MD, PhD, Department of Gastroenterology, Clinic of Internal Medicine, University Hospital of North Norway, Sykehusveien, N-9038 Tromso, Norway; Salvatore Auricchio, MD, PhD, Professor, Scientific Director of European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Via S. Pansini 5, I-80131 Naples, Italy
S- Editor Gou SX L- Editor A E- Editor Zhang DN
References
1.
Catassi C, Fabiani E, Rätsch IM, Coppa GV, Giorgi PL, Pierdomenico R, Alessandrini S, Iwanejko G, Domenici R, Mei E. The coeliac iceberg in Italy. A multicentre antigliadin antibodies screening for coeliac disease in school-age subjects. Acta Paediatr Suppl. 1996;412:29-35.[PubMed] [DOI]
2.
Fasano A, Berti I, Gerarduzzi T, Not T, Colletti RB, Drago S, Elitsur Y, Green PH, Guandalini S, Hill ID. Prevalence of celiac disease in at-risk and not-at-risk groups in the United States: a large multicenter study. Arch Intern Med. 2003;163:286-292.[PubMed] [DOI]
3.
Mäki M, Collin P. Coeliac disease. Lancet. 1997;349:1755-1759.[PubMed] [DOI]
4.
Dicke WK. [Treatment of celiac disease]. Ned Tijdschr Geneeskd. 1951;95:124-130.[PubMed]
5.
Hogen Esch CE, Wolters VM, Gerritsen SA, Putter H, von Blomberg BM, van Hoogstraten IM, Houwen RH, van der Lely N, Mearin ML. Specific celiac disease antibodies in children on a gluten-free diet. Pediatrics. 2011;128:547-552.[PubMed] [DOI]
6.
Rostom A, Dubé C, Cranney A, Saloojee N, Sy R, Garritty C, Sampson M, Zhang L, Yazdi F, Mamaladze V. The diagnostic accuracy of serologic tests for celiac disease: a systematic review. Gastroenterology. 2005;128:S38-S46.[PubMed] [DOI]
7.
Paulley JW. Observation on the aetiology of idiopathic steatorrhoea; jejunal and lymph-node biopsies. Br Med J. 1954;2:1318-1321.[PubMed]
8.
Mubarak A, Nikkels P, Houwen R, Ten Kate F. Reproducibility of the histological diagnosis of celiac disease. Scand J Gastroenterol. 2011;46:1065-1073.[PubMed] [DOI]
9.
Ravelli A, Villanacci V, Monfredini C, Martinazzi S, Grassi V, Manenti S. How patchy is patchy villous atrophy?: distribution pattern of histological lesions in the duodenum of children with celiac disease. Am J Gastroenterol. 2010;105:2103-2110.[PubMed] [DOI]
10.
Pais WP, Duerksen DR, Pettigrew NM, Bernstein CN. How many duodenal biopsy specimens are required to make a diagnosis of celiac disease?. Gastrointest Endosc. 2008;67:1082-1087.[PubMed] [DOI]
11.
Hill PG, Forsyth JM, Semeraro D, Holmes GK. IgA antibodies to human tissue transglutaminase: audit of routine practice confirms high diagnostic accuracy. Scand J Gastroenterol. 2004;39:1078-1082.[PubMed] [DOI]
12.
Hopper AD, Hadjivassiliou M, Hurlstone DP, Lobo AJ, McAlindon ME, Egner W, Wild G, Sanders DS. What is the role of serologic testing in celiac disease? A prospective, biopsy-confirmed study with economic analysis. Clin Gastroenterol Hepatol. 2008;6:314-320.[PubMed] [DOI]
13.
Hadithi M, von Blomberg BM, Crusius JB, Bloemena E, Kostense PJ, Meijer JW, Mulder CJ, Stehouwer CD, Peña AS. Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease. Ann Intern Med. 2007;147:294-302.[PubMed]
14.
Reeves GE, Squance ML, Duggan AE, Murugasu RR, Wilson RJ, Wong RC, Gibson RA, Steele RH, Pollock WK. Diagnostic accuracy of coeliac serological tests: a prospective study. Eur J Gastroenterol Hepatol. 2006;18:493-501.[PubMed] [DOI]
15.
Husby S, Koletzko S, Korponay-Szabó IR, Mearin ML, Phillips A, Shamir R, Troncone R, Giersiepen K, Branski D, Catassi C. European Society for Pediatric Gastroenterology, Hepatology, and Nutrition guidelines for the diagnosis of coeliac disease. J Pediatr Gastroenterol Nutr. 2012;54:136-160.[PubMed] [DOI]
16.
Hill PG, Holmes GK. Coeliac disease: a biopsy is not always necessary for diagnosis. Aliment Pharmacol Ther. 2008;27:572-577.[PubMed] [DOI]
17.
Vivas S, Ruiz de Morales JG, Riestra S, Arias L, Fuentes D, Alvarez N, Calleja S, Hernando M, Herrero B, Casqueiro J. Duodenal biopsy may be avoided when high transglutaminase antibody titers are present. World J Gastroenterol. 2009;15:4775-4780.[PubMed] [DOI]
18.
Dahlbom I, Korponay-Szabó IR, Kovács JB, Szalai Z, Mäki M, Hansson T. Prediction of clinical and mucosal severity of coeliac disease and dermatitis herpetiformis by quantification of IgA/IgG serum antibodies to tissue transglutaminase. J Pediatr Gastroenterol Nutr. 2010;50:140-146.[PubMed] [DOI]
19.
Bonamico M, Thanasi E, Mariani P, Nenna R, Luparia RP, Barbera C, Morra I, Lerro P, Guariso G, De Giacomo C. Duodenal bulb biopsies in celiac disease: a multicenter study. J Pediatr Gastroenterol Nutr. 2008;47:618-622.[PubMed] [DOI]
20.
Marsh MN. Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity (‘celiac sprue’). Gastroenterology. 1992;102:330-354.[PubMed]
21.
Oberhuber G. Histopathology of celiac disease. Biomed Pharmacother. 2000;54:368-372.[PubMed] [DOI]
22.
Barker CC, Mitton C, Jevon G, Mock T. Can tissue transglutaminase antibody titers replace small-bowel biopsy to diagnose celiac disease in select pediatric populations?. Pediatrics. 2005;115:1341-1346.[PubMed] [DOI]
23.
Donaldson MR, Firth SD, Wimpee H, Leiferman KM, Zone JJ, Horsley W, O’Gorman MA, Jackson WD, Neuhausen SL, Hull CM. Correlation of duodenal histology with tissue transglutaminase and endomysial antibody levels in pediatric celiac disease. Clin Gastroenterol Hepatol. 2007;5:567-573.[PubMed] [DOI]
24.
Mubarak A, Wolters VM, Gerritsen SA, Gmelig-Meyling FH, Ten Kate FJ, Houwen RH. A biopsy is not always necessary to diagnose celiac disease. J Pediatr Gastroenterol Nutr. 2011;52:554-557.[PubMed] [DOI]
25.
Donaldson MR, Book LS, Leiferman KM, Zone JJ, Neuhausen SL. Strongly positive tissue transglutaminase antibodies are associated with Marsh 3 histopathology in adult and pediatric celiac disease. J Clin Gastroenterol. 2008;42:256-260.[PubMed] [DOI]
26.
Freeman HJ. Strongly positive tissue transglutaminase antibody assays without celiac disease. Can J Gastroenterol. 2004;18:25-28.[PubMed]
27.
Fernández-Bañares F, Alsina M, Modolell I, Andújar X, Piqueras M, García-Puig R, Martín B, Rosinach M, Salas A, Viver JM. Are positive serum-IgA-tissue-transglutaminase antibodies enough to diagnose coeliac disease without a small bowel biopsy? Post-test probability of coeliac disease. J Crohns Colitis. 2012;6:861-866.[PubMed] [DOI]