Effect of itopride, a new prokinetic, in patients with mild GERD: A pilot study

Abstract

AIM: Itopride is a newly developed prokinetic agent, which enhances gastric motility through both antidopaminergic and anti-acetylcholinesterasic actions. The importance of esophageal motor dysfunction in the pathogenesis of gastro-esophageal reflux disease (GERD) makes it interesting to examine the effect of itopride on esophageal acid exposure.

METHODS: The effect of itopride on esophageal acid reflux variables for 24 h was studied in 26 patients with GERD symptoms, pre-entry total acid exposure time (pH<4) of more than 5% and mild esophagitis (Savary-Miller grades I, II) proven by endoscopy. Ambulatory 24-h pH-metry and symptom assessment were performed after treatments with 150 or 300 mg itopride thrice a day (t.i. d.) for 30 d in random order, using an open label method. For evaluating the safety of itopride, blood biochemical laboratory test was performed and the serum prolactin level was also examined before and after treatment.

RESULTS: Total symptom score was significantly decreased after treatment in 150- or 300-mg group. Itopride 300 mg was significantly effective than 150 mg on decreasing the total per cent time with pH < 4, total time with pH < 4 and DeMeester score. No serious adverse effects were observed with administration of itopride in both groups.

CONCLUSION: Itopride 100 mg t.i.d. is effective on decreasing pathologic reflux in patient with GERD and therefore it has the potential to be effective in the treatment of this disease.

Key Words: Gastro-esophageal reflux disease, Itopride

INTRODUCTION

Gastro-esophageal reflux disease (GERD) is characterized by the reflux of gastric content into esophagus with or without histological changes. However, the excessive reflux of gastric acid induces some complications such as esophagitis, esophageal stenosis, cancer, or Barrett’s esophagus. Pathogenesis of GERD are lower esophageal sphincter (LES) dysfunction, abnormal clearing capacity of refluxed materials, delayed gastric emptying and abnormal resistance of esophageal mucosa to gastric acid, but the primary motor dysfunction is regarded as the most important factor in general[1,2]. Therefore, prokinetic agent that restores gastric motility with increasing of LES and esophageal motility has been developed and used frequently in the treatment of GERD[3]. There are several prokinetic agents such as bethanechol, metoclopramide, domperidone, and cisapride that potentiate acetylcholine effects on smooth muscle of gastrointestinal tract or antagonize the activity of dopamine.

However, these prokinetic agents have some limitations for prescribing to GERD patients, because they show uncertain efficacies on GERD except for cisapride and have some problems in safety[4,5]. Especially, cisapride accelerates the esophageal acid clearance by an enhancement of esophageal propulsive motility, which strengthens LES and stimulates the gastric emptying. So it is regarded that cisapride has the same efficacy as an inhibitor of gastric acid secretion for GERD. In spite of these predominant effects, cisapride is restricted to use because of a safety problem that it provokes arrhythmia.

Itopride, a new type of the prokinetic agent, acts both as a dopamine D₂ receptor antagonist and as an acetylcholine esterase inhibitor. It accelerates gastric emptying, improves gastric tension and sensitivity, and has an anti-emetic action. And also, itopride was identified to have equivalent efficacy with cisapride in functional dyspepsia. These mechanisms of itopride may be useful to GERD patients, but no clinical data are available from them as yet.

The aim of this pilot study is to evaluate the efficacy of itopride for the treatment of GERD by evaluating the improvement of the clinical symptom and esophageal pH change.

MATERIALS AND METHODS

Study design

This study was performed prospectively to evaluate the efficacy and dose-response of itopride for the treatment of GERD by estimating ambulatory 24-h pH-metry and clinical symptoms before and after treatment. Patients satisfying the entry criteria were assigned to two groups by the randomization allocation schedule and received either 150 or 300 mg of itopride per day.

Inclusion/exclusion criteria

Twenty-six patients (range 18-65 years) who visited the Department of Internal Medicine in Wonkwang University and agreed to participate in this study were recruited based on the following entry criteria: Inclusion criteria were (1) more than moderate heartburn symptom; (2) more than one upper dyspeptic symptoms such as regurgitation, epigastric pain, nausea, vomiting, dysphagia, chest pain lasting for more than 4 wk; (3) less than grade II esophagitis by Savary-Miller classification by endoscopic examination; (4) abnormal acid exposure on 24-h pH-metry (fraction of time of >5% with pH below 4).

For each symptom, the severity scores are as follows: 0 (none) = absent; 1 (mild) = symptom was not spontaneously reported but elicited upon specific questions; 2 (moderate) = symptom needs to be treated but not interfered with normal activities; 3 (severe) = symptom interfered with normal activities; 4 (very severe) = impossible to do normal activities due to symptom.

Exclusion criteria were the following: (1) acute esophagitis related to strong stimuli; (2) corrosive esophagitis by a toxicant; (3) esophagitis or some symptoms by inflammatory infection or radiotherapy; (4) regular use of H₂ blockers, prokinetic or anticholinergic agents for previous 4 wk; (5) previous gastrointestinal surgery; (6) inflammatory bowel disease; (7) cardiological, respiratory, gastrointestinal disease, endocrine metabolic disease and neuro-psychological disease; (8) clinically significant hepatic or renal dysfunction; (9) pregnancy or lack of adequate contraception in the case of women at child-bearing age; (10) patients who are inappropriate to this study by investigator’s opinion.

Study procedures

This study was approved by the institutional review board of Wonkwang University Hospital and informed consent was obtained from patients for inclusion in this study. The patients were asked about basic information and previous treatment history. And they were screened as physical examination, vital sign and lab examination. Any drugs that could influence the study were postponed for at least 1 wk before that examination.

The baseline symptom of GERD was evaluated by questionnaire. Endoscopic examination was performed to check the organic lesion like cancer or ulcer, and to evaluate the severity of esophagitis. After examination, ambulatory 24-h pH-metry was carried out.

Patients who met the entry criteria were allocated randomly to 150- or 300-mg group of itopride. Fifty or 100 mg of itopride was administered thrice per day for 4 wk. After treatment of itopride for 4 wk, physical examination, vital sign, laboratory test, and symptoms of GERD were evaluated and ambulatory 24-h pH-metry was performed.

Efficacy parameters were (1) changes in acid reflux episodes measured by ambulatory pH-metry such as number of reflux episodes, number of reflux episodes lasting for more than 5 min, duration of longest episodes, % time with pH < 4, and DeMeester score; (2) changes in subjective symptom of patient with GERD.

Safety parameters were (1) results of laboratory test (biochemistry, complete blood count, urinalysis and prolactin level); (2) occurrences of adverse events after the treatment. If adverse events occurred, the time of onset, duration, severity, relationship to itopride and the requirement of treatment were evaluated.

Statistical analysis

Two sample t-test and paired t-test were used for continuous variables presented as a normal distribution; Wilcoxon signed rank test and Wilcoxon rank sum test were used for those not presented as a normal distribution. And χ² test or Fisher’s exact test was used for categorical variables. All data were statistically analyzed with SAS package (version 8.0) and differences with a value of P < 0.05 were considered significant.

RESULTS

Patients’ characteristics

The mean age and body weight of patients in 150-mg group were 44 ± 9 years and 64 ± 11 kg, those of patients in 300-mg group were 45 ± 12 years and 61 ± 12 kg. Seven were men, six were women in 150-mg group and five were men, eight were women in 300-mg group.

Baseline characteristics of patients in two groups are detailed in Table 1 and no statistical differences were noted in the past history, smoking and drinking history and endoscopic results.

Table 1 Clinical characteristics of patients.

	150 mg		300 mg	P
Age (yr)		44 ± 9	45 ± 12	0.8141
Sex	Male	7	5	0.734
	Female	6	8
Weight		64 ± 11	61 ± 12	0.4281
Duration of reflux disease	< 6 mo	5	2	0.412
	6 -2 yr	6	8
	> 2 yr	2	3
Pre-medication	Yes	2	1	0.828
	No	11	12
Endoscopy	0	5	7	0.717
(Savary-Miller grade)
	I	7	5
	II	1	1
Drinking	Yes	2	3	0.884
	No	11	10
Smoking	Yes	3	3	1
	No	10	10

P value by χ² test except for ¹two sample t-test.

Efficacy of itopride on GERD symptoms

A significant improvement of all symptoms including heartburn, a main symptom of GERD, was identified after the 150 and 300 mg of itopride treatment compared with the pre-treatment (Figure 1A). No statistically significant differences were found between two groups in the symptom improvement (Figure 2B).

Figure 1 A: Change in mean heartburn score after treatment of itopride. The heartburn score of patients with GERD was improved in both groups of itopride significantly. ^bP < 0.0001, ¹P = 0.0006 vs pretreatment by paired t-test; B: Change in mean total symptom score after treatment of itopride. The mean total symptom score of patients with GERD was improved in both groups of itopride significantly. ^dP < 0.0001 vs pretreatment by paired t-test.

Figure 2 Change in mean number of acid refluxes after itopride treatment. Mean number of acid refluxes was significantly improved in 300-mg group. ¹P = 0.057 vs pretreatment by Wilcoxon’s signed rank test.

Efficacy of itopride on esophageal acid reflux variables

Among the acid reflux variables recorded, total time of pH < 4, per cent time with pH<4 and DeMeester score were significantly improved in 300-mg group compared with the pre-treatment (Figure 2). In 150-mg group, itopride significantly reduced the duration of longest episode compared with the pre-treatment. The individual values of acid reflux variable before and after treatment with 150 or 300 mg of itopride are given in Table 2.

Table 2 Changes of acid reflux variables and DeMeester score in 26 patients with GERD after treatment with 150 or 300 mg of itopride.

Variable	Group (mg/d)	Itopride Tx.	n	Mean	SD	P
Number of acid refluxes	150	Pre	12	99.25	88.57	0.97
		Post	12	76.92	60.53
	300	Pre	13	150.77	246.15	0.057
		Post	13	52.23	39.79
Number of acid refluxes longer than 5 min	150	Pre	12	6.08	5.32	0.287
		Post	12	3.83	5.32
	300	Pre	13	6.23	5.12	0.054
		Post	13	2.69	2.5
Longest time acid refluxes (min)	150	Pre	12	30.55	33.37	0.018
		Post	12	14.09	11.03
	300	Pre	13	30.25	56.4	0.289
		Post	13	20.78	33.64
Total time below pH 4 (min)	150	Pre	12	138.42	98.56	0.16
		Post	12	92.09	78.07
	300	Pre	13	145.08	86.1	0.011
		Post	13	71.77	51.43
Fraction time below pH 4 (%)	150	Pre	12	11.13	9.26	0.211
		Post	12	10.12	12.69
	300	Pre	13	11.2	6.69	0.005
		Post	13	5.27	3.57
DeMeester score	150	Pre	12	32.5	16.96	0.16
		Post	12	22.89	18.75
	300	Pre	13	44.48	37.5	0.007
		Post	13	19.56	14.72

Safety of itopride

Itopride was well tolerated and only minor adverse events were reported. There were no significant changes in the mean level of prolactin with the 4-wk treatment of 150- or 300-mg itopride (Figure 3). In two patients of the 300-mg group, prolactin level was increased over 15 ng/mL, but there were no adverse events like lacteal secretion.

Figure 3 Change in mean level of prolactin after itopride treatment. The level of prolactin was not changed after treatment of itopride in GERD.

DISCUSSION

The result of this pilot study demonstrates that itopride decreases esophageal acid reflux and relieved symptoms without any significant adverse event in patients with GERD. These results show that itopride can improve the pathologic conditions of GERD.

The aims of treatment for GERD are relief of symptoms and healing of damaged lesion if endoscopic or pathologic examinations revealed mucosal damage of esophagus. Currently, it is being considered that long-term treatment is required because GERD has been regarded as chronic-relapsing disease like hypertension and diabetes mellitus[12,13].

Among several therapeutic drugs, a prokinetic agent has been used for the treatment and improvement of pathogenic mechanism of GERD such as gastrointestinal motility disorder, incompetent LES relaxation, impaired esophageal acid clearance, and prolonged gastric emptying. Among the kinds of prokinetic drugs, many studies have focused on the effect of cisapride on GERD. In one of them, cisapride increased tones of esophageal peristalsis and LES dose-dependency in healthy subjects[14,15]. On the contrary, in the other study, cisapride did not have any influence on the postprandial esophageal peristalsis, gastric acid[16] and LES pressure[17] in healthy subjects.

Even though this study does not confirm the efficacy of itopride on the esophageal peristalsis and LES, considering that itopride improves symptoms of the patients with GERD and decreases acid reflux effectively, we can speculate that itopride exerts some effects on improving esophageal motor dysfunction. Further studies will be required for making out the effect of itopride on the esophageal motor dysfunction of GERD.

From the viewpoint of improving symptom and healing mucosa, it was observed that administration of cisapride 10 mg four times a day (q.i.d.) and 20 mg twice a day (b.i. d.) improved the esophageal reflux compared with placebo, and cisapride had the same effect as H₂ blocker on treatment of patients with mild esophagitis[6,7]. Additionally, it was reported that a combination therapy with cisapride and H₂ blocker was more effective than monotherapy in patients with severe reflux esophagitis[18].

Although a few studies on functional dyspepsia show that the efficacy of itopride was equivalent to that of cisapride[11], this is the first study reporting that administration of itopride for 4 wk improves symptoms of GERD including heartburn (the main symptom of GERD) in both 150- and 300-mg group. So hereafter, additional studies will be required for evaluating the effect of itopride monotherapy or combination therapy with H₂ receptor inhibitors on the esophagitis with mucosal damage as the initial and maintenance treatment.

The most standard method to measure the variation of esophageal acid is ambulatory 24-h pH-metry. In other studies using a similar method, cisapride 10 mg q.i.d. for 8 wk was more effective than placebo in terms of decreasing the number of reflux episodes, duration of longest episode, and percent time with pH < 4. These results suggest that cisapride is useful for acid reflux in patients with GERD[19].

In the study of mosapride 40 mg every day (q.d.) for 2 d, it reduced the number of reflux episodes, the number of reflux episodes > 5 min, and percent time with pH<4 and improved acid clearance time[20]. In this study, itopride 100 mg t.i.d. for 4 wk was effective in decreasing percent time with pH < 4 and DeMeester score. In this regard, it can be considered that itopride has a similar effect on decreasing acid reflux as cisapride or mosapride, although it is different in the action mechanism or duration of treatment from cisapride or mosapride[9,10].

The action mechanism of itopride on the reflux variables is unclear so far. But, dual mechanism of itopride, acetylcholine esterase inhibitory action and antagonistic activity on dopamine D₂ receptor, which is known to inhibit activities of acetylcholine[21], seems to make this drug effective on promoting the esophageal and gastrointestinal movement and on improving acid clearance. It has been known that the threshold of itopride to promote gastrointestinal motility is higher than that of cisapride, metoclopramide and domperidone[8].

Prokinetic drugs that inhibit dopamine D₂ receptor may elicit some adverse events such as fatigue, confusion, extrapyramidal symptom, and hyperprolactinemia that cause lacteal secretion or breast enlargement. Although there was no extensive study concerned about safety of itopride, the development of extrapyramidal symptom or hyperprolactinemia was uncommon with the administration of usual dosage of itopride (150 mg/d). In this study, there was no hyperprolactinemia and extrapyramidal symptoms in 150-mg group of itopride and there were two cases showing over 15 ng/mL of prolactin level in 300-mg group of itopride without adverse events like lacteal secretion[22].

Taken together, these results demonstrate that the administration of 150 or 300 mg of itopride for 4 wk improves symptoms of GERD and that the administration of 300 mg of itopride for 4 wk improves mild esophagitis and pathologic acid reflux. Therefore, itopride can be useful for treatment of the patients with GERD.