Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 21, 2017; 23(19): 3427-3439
Published online May 21, 2017. doi: 10.3748/wjg.v23.i19.3427
Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line
Nandakumar Packiriswamy, Kari F Coulson, Susan J Holcombe, Lorraine M Sordillo
Nandakumar Packiriswamy, Kari F Coulson, Susan J Holcombe, Lorraine M Sordillo, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, United States
Author contributions: Packiriswamy N, Coulson KF, Holcombe SJ and Sordillo LM designed the study; Packiriswamy N, Coulson KF and Holcombe SJ performed the experiments; Packiriswamy N, Coulson KF, Holcombe SJ, and Sordillo LM interpreted the data, prepared the manuscript, and approved the final version of the article to be published.
Supported by an endowment from the Matilda R. Wilson Fund in Detroit, Michigan.
Institutional animal care and use committee statement: The Animal Use and Care Committee at Michigan State University approved the protocols used in this study.
Conflict-of-interest statement: None of the authors have conflicts of interest to report.
Data sharing statement: No additional data are available for sharing.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Lorraine M Sordillo, Professor, Meadow Brook Chair, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 784 Wilson Road, East Lansing, MI 48824, United States. sordillo@msu.edu
Telephone: +1-517-4328821 Fax: +1-517-4328822
Received: December 12, 2016
Peer-review started: December 14, 2016
First decision: December 29, 2016
Revised: January 13, 2017
Accepted: March 15, 2017
Article in press: March 15, 2017
Published online: May 21, 2017
Core Tip

Core tip: The normal human colon cell line, CRL.1970, can recapitulate oxidative stress-induced responses associated with inflammatory bowel disease following microbial challenge including enhanced production of reactive oxygen species (ROS), inflammatory cytokines, and enhanced mitochondrial autophagic responses. Scavenging mitochondrial ROS inhibited mitochondrial morphologic changes and autophagy suggesting that CRL.1790 cells can be used to study oxidative events associated with intestinal inflammatory disorders.