Copyright
©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 28, 2016; 22(24): 5532-5539
Published online Jun 28, 2016. doi: 10.3748/wjg.v22.i24.5532
Published online Jun 28, 2016. doi: 10.3748/wjg.v22.i24.5532
MicroRNA-21 promotes phosphatase gene and protein kinase B/phosphatidylinositol 3-kinase expression in colorectal cancer
Wei-Zhong Sheng, Yu-Sheng Chen, Bo Zhang, Wei-Dong Gao, Department of General Surgery, Fudan University affiliated Zhongshan Hospital, Shanghai 200032, China
Chuan-Tao Tu, Juan He, Department of Digestion, Fudan University affiliated Zhongshan Hospital, Shanghai 200032, China
Author contributions: Sheng WZ and Chen YS contributed equally to this study, Sheng WZ, Chen YS and Gao WD designed research; Sheng WZ, Chen YS, Gao WD, Tu CT, He J and Zhang B performed research; Gao WD, Tu CT, He J and Zhang B contributed new reagents or analytic tools; Sheng WZ and Chen YS analyzed data; Sheng WZ and Gao WD wrote the paper.
Institutional review board statement: The study was reviewed and approved by the Fudan University affiliated Zhongshan Hospital Institutional review board.
Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Wei-Dong Gao, MD, Associate Professor, Department of General Surgery, Fudan University affiliated Zhongshan Hospital, NO. 180 Fenglin Road, Shanghai 200032, China. gao.weidong@zs-hospital.sh.cn
Telephone: +86-21-64041990 Fax: +86-21-64041990
Received: February 19, 2016
Peer-review started: February 20, 2016
First decision: March 21, 2016
Revised: April 11, 2016
Accepted: May 4, 2016
Article in press: May 4, 2016
Published online: June 28, 2016
Peer-review started: February 20, 2016
First decision: March 21, 2016
Revised: April 11, 2016
Accepted: May 4, 2016
Article in press: May 4, 2016
Published online: June 28, 2016
Core Tip
Core tip: RT-PCR and Western blot were applied to detect the expression level of microRNA-21 (miR-21) and Phosphatase gene (PTEN), including its downstream proteins protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K) in colorectal cancer (CRC) cell lines, and to explore the regulatory mechanism of the expression of miR-21 in inhibiting CRC, respectively. Their associations were investigated to clarify whether one of the direct target genes of miR-21 is PTEN. The expression levels of miR-21, PTEN and its downstream proteins AKT and PI3K are regulated and controlled to manage the occurrence and progression of CRC.