Wang R.Early diagnosis of hepatitis A virus-micro-blotting immuno-binding assay. World J Gastroenterol 1998 October;4(Suppl 2):tk74b-75
Early diagnosis of hepatitis A virus-micro-blotting immuno-binding assay
Wang R.
Department of Scientific Research, Beijing Friendship Hospital,
Beijing 100050, China
AIM:To establish a quick and easy method for detecting the antI-HAV IgM level in sera of HAV patients during acute periods.METHODS To use micro-blot immuno-binding assay (MBIMA) was used to detect the titration of Goat-anti-Human(GaH) IgM-HRP and titration of IgM in sera of healthy people. RIA method and MBIBA methods were used to detect the IgM level in sera in 114 HAV patients and 48 non-HAV patients. RESULTS Titration of IgM and GaH IgM-HRP in healthy people were 1:1000 and 1:1600, respectively; The IgM level in sera in 114 patients was positive by RIA method and that in 112 patients was positive by MBIBA method; the IgM level in sera in 48 non-HAV people was negative by both methods; the duration of RIA and MBIBA were 2-day and 6-hour, respectively.CONCLUSION:The positive and negative coincidence rates of RIA and MBIBA in detecting the serum IgM level in patients were 98.24% and 100%, respectively; the MBIBA method was much easier and quicker than RIA and might be used widely in clinical practice.
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