World J Gastroenterol 2010 July 14; 16(26): 3292-3298
World J Gastroenterol. 2010 July 14; 16(26): 3292-3298.
Published online 2010 July 14. doi: 10.3748/wjg.v16.i26.3292.
Copyright©2010 Baishideng. All rights reserved.
Functional magnetic resonance imaging in an animal model of pancreatic cancer
Robert J Lewandowski, Aaron C Eifler, David J Bentrem, Johnathan C Chung, Dingxin Wang, Gayle E Woloschak, Guang-Yu Yang, Robert Ryu, Riad Salem, Andrew C Larson and Reed A Omary.
Robert J Lewandowski, Aaron C Eifler, Johnathan C Chung, Dingxin Wang, Robert Ryu, Riad Salem, Andrew C Larson, Reed A Omary, Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, United States
David J Bentrem, Department of Surgical Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, United States
Gayle E Woloschak, Department of Radiation Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, United States
Guang-Yu Yang, Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, United States
Author contributions: Larson AC, Omary RA and Lewandowski RJ designed the research; Bentrem DJ, Woloschak GE and Yang GY contributed the animal model; Chung JC, Wang D and Larson AC performed the imaging; Ryu R, Salem R and Omary RA performed catheterization of rabbits; Eifler AC analyzed the data; Lewandowski RJ and Eifler AC wrote the manuscript.
Correspondence to: Robert J Lewandowski, MD, Assistant Professor, Department of Radiology, Northwestern University Feinberg School of Medicine, 676 N St Clair St, Suite 800, Chicago, IL 60611, United States. r-lewandowski@northwestern.edu
Telephone: +1-312-6959121 Fax: +1-312-9265991
Received November 25, 2009; Revised December 24, 2009; Accepted December 31, 2009;
This article has been cited by other articles in PMC.
Abstract
AIM: To test the hypotheses that diffusion weighed (DW)- and transcatheter intraarterial perfusion (TRIP)-magnetic resonance imaging (MRI) can each be used to assess regional differences in tumor function in an animal pancreatic cancer model.
METHODS: VX2 tumors were implanted in pancreata of 6 rabbits. MRI and digital subtraction angiography (DSA) were performed 3 wk following implantation. With a 2-French catheter secured in the rabbit’s gastroduodenal artery, each rabbit was transferred to an adjacent 1.5T MRI scanner. DW- and TRIP-MRI were performed to determine if necrotic tumor core could be differentiated from viable tumor periphery. For each, we compared mean differences between tumor core/periphery using a 2-tailed paired t-test (α = 0.05). Imaging was correlated with histopathology.
RESULTS: Tumors were successfully grown in all rabbits, confirmed by necropsy. On DW-MRI, mean apparent diffusion coefficient (ADC) value was higher in necrotic tumor core (2.1 ± 0.3 mm2/s) than in viable tumor periphery (1.4 ± 0.5 mm2/s) (P < 0.05). On TRIP-MRI, mean perfusion values was higher in tumor periphery (110 ± 47 relative units) than in tumor core (66 ± 31 relative units) (P < 0.001).
CONCLUSION: Functional MRI can be used to differentiate necrotic from viable tumor cells in an animal pancreatic cancer model using ADC (DW-MRI) and perfusion (TRIP-MRI) values.
Keywords: Animal model, Functional magnetic resonance imaging, Pancreatic cancer