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World J Gastroenterol. Dec 28, 2008; 14(48): 7386-7391
Published online Dec 28, 2008. doi: 10.3748/wjg.14.7386
Inhibitory effect of modified citrus pectin on liver metastases in a mouse colon cancer model
Hai-Ying Liu, Zhi-Liang Huang, Guo-Hua Yang, Wei-Qun Lu, Nan-Rong Yu
Hai-Ying Liu, Zhi-Liang Huang, Guo-Hua Yang, Wei-Qun Lu, Nan-Rong Yu, Department of Abdominal Surgery, Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, Guangdong Province, China
Author contributions: Liu HY designed the research; Huang ZL, Yang GH, Lu WQ and Yu NR performed the research; Liu HY and Huang ZL analyzed the data and wrote the paper.
Correspondence to: Hai-Ying Liu, MD, PhD, Department of Abdominal Surgery, Affiliated Tumor Hospital of Guangzhou Medical College, #78 Hengzhigang Road, Guangzhou 510095, Guangdong Province, China. gzdlhy@yahoo.com.cn
Telephone: +86-20-83595032-3129 Fax: +86-20-83598205
Received: September 3, 2008
Revised: November 25, 2008
Accepted: December 2, 2008
Published online: December 28, 2008
Abstract

AIM: To discuss the expression of glactin-3 in liver metastasis of colon cancer and its inhibition by modified citrus pectin (MCP) in mice.

METHODS: Seventy-five Balb/c mice were randomly divided into negative control group (n = 15), positive control group (n = 15), low MCP concentration group (n = 15), middle MCP concentration group (n = 15) and high MCP concentration group (n = 15). CT26 colon cancer cells were injected into the subcapsule of mouse spleen in positive control group, low, middle and high MCP concentrations groups, except in negative control, to set up a colon cancer liver metastasis model. The concentration of MCP in drinking water was 0.0%, 0.0%, 1.0%, 2.5% and 5.0% (wt/vol), respectively. Liver metastasis of colon cancer was observed after 3 wk. Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of galectin-3 in serum. Expression of galectin-3 in liver metastasis was detected by immunohistochemistry.

RESULTS: Except for the negative group, the percentage of liver metastasis in the other 4 groups was 100%, 80%, 73.3% and 60%, respectively. The number of liver metastases in high MCP concentration group was significantly less than that in positive control group (P = 0.008). Except for the negative group, the median volume of implanted spleen tumor in the other 4 groups was 1.51 cm3, 0.93 cm3, 0.77 cm3 and 0.70 cm3, respectively. The volume of implanted tumor in middle and high MCP concentration groups was significantly smaller than that in positive control group (P = 0.019; P = 0.003). The concentration of serum galectin-3 in positive control and MCP treatment groups was significantly higher than that in the negative control group. However, there was no significant difference between them. Except for the negative control group, the expression of galectin-3 in liver metastases of the other 4 groups showed no significant difference.

CONCLUSION: Expression of galectin-3 increases significantly in liver metastasis of colon cancer, which can be effectively inhibited by MCP.

Keywords: Pectin, Colonic neoplasms, Metastasis, Liver, Mice