|
Yan
Huang1, Shi Jun Lu2, Jing Xia Dong3 and Feng
Li3
1Department
of Histology and Embryology, 2Department of Pathology,
Weifang Medical College, Weifang 261042
3Department of Histology and Embryology, Beijing Medical
University, Beijing 100083, China
Dr. Yan Huang, graduated from Beijing Medical University as a Ph.D
in 1995, now professor and postgraduate tutor, majoring in
neuroendocrine research,
having 18 papers published.
Supported by the Foundation of Shandong Educational Committee
Correspondence to: Yan Huang, Department of Histology and
Embryology,Weifang Medical College,Weifang 261042, Shandong
Province, China
Telephone:
0086-536-8271378
Email. yphyyt@wf_public.sd.cninfo.net
Received: 2000-01-18 Accepted: 2000-03-14
Subject
headings: peptic
ulcer; plasma cells; gastric mucosa; islet amyloid polypeptide (IAPP);
neuro-endocrine-immune network
Huang
Y, Lu SJ, Dong JX, Li F. The new proof of neuro-endocrine-immune
network—expression
of islet amyloid polypeptide in plasma cells in gastric mucosa of
peptic ulcer patients. World J Gastroentero, 2000;6(3):417-418
INTRODUCTION
Peptic ulcer, as a common disease, seriously affected people＇s,
work and life. Its occurrence, development and change have close
relationship with the change of people＇s
moods. Animal experiment proved that significant changes occurred in
the endocrine system of the gastric ulcer rats［1］.
Recent study also showed that the number of lymphocytes increased
markedly in the gastric mucosa of peptic ulcer patients［2］.
All the above indicated
that peptic ulcer is closely related neuro-endocrine-immune system.
IAPP, a novel islet hormone, not only takes part in the regulation
of blood glucose［3］,
but also protects gastric mucosa［4］and
regulates gastrointestinal movements［5］.
On the basis of previous studies, we observed the expression change
of IAPP and explored the relationship between the endocrin
e and the immune system in gastric mucosa of peptic ulcer patients,
so as to provide morphologic data on the existence of neuro-endocrine-immune
network and the changes in peptic ulcer.
MATERIALS AND METHODS
Twenty-one samples, including 6 cases from normal human stomach,
15 cases from
gastrectomy of gastric ulcer patients, were collected. The paraffin
sections were prepared as usual. Immunohistochemical PAP method was
used to show IAPP-IR cells. Briefly five-micron sections were placed
on glass slides deparaffinized in xylene, rinsed in ethanol, and
brought to PBS through a series of descending concentration of
ethanol; endogenous peroxidase activity was blocked with methanol-H2O2
at room temperature for 30min; rabbit anti-IAPP serum (peninsula,
USA) was diluted 1/6000 with PBS, and the sections were incubated
overnight at 4℃.
Goat anti-rabbit IgG (Huamei, Beijing) (1/50),
peroxidase-anti-peroxidase (Capital Medical University) (1/100) and
DAB kit (Zhongshan, Beijing) were used for staining. As the negative
control, the primary antiserum was replaced by PBS and other steps
were the same as stated above. All the sections were counterstained
with Mayer hematoxylin.
RESULTS
The IAPP-IR cell was not observed in the gastric mucosa of
normal subject (Figure 1A). In comparison, a great number of plasma
cells IAPP-IR were found
in the gastric mucosa of peptic ulcer patients (Figure 1B,C). Most
of IAPP-IR p
lasma cells were weak and only a few were strong for IAPP staining
(Figure 1D). Of the negative control sections, no immunoreactive
product to IAPP was found in plasma cells (Figure 1E).
DISCUSSION
The gastric mucosa, in which there are a lot of neurons,
endocrine cells and immunocytes that may interact with each other,
is an important field for the study of neuro-endocrine-immune
network. It will undoubtedly provide valuable data for the study on
this network by exploring the change of immune-endocrine of gastric
mucosa of peptic ulcer patients. Based on the observation of T and B
lymphocytes which increase obviously in the gastric mucosa of peptic
ulcer patients［2］and
the action of IAPP, a novel islet hormone, which inhibits gastric
acid secretion［6］and
protects gastric mucosa［4］,
we further studied the expression change of IAPP in the gastric muc
osa. Unexpectedly, it was found that the plasma cells of gastric
mucosa increase
d in number, moreover most of them expressed IAPP to some degree.
Figure 1 Immunohistochemical PAP method, Mayer Hematoxylin
counterstained showing IAPP-IR cells.
A. The gastric
mucosa of normal human, no IAPP-IR cell was found. ×200
B-E. The gastric mucosa of peptic ulcer patients.
B. The more IAPP IR
plasma cells (↑).
×1000
C. The weak
positive (↑)
and negative (▲)
plasma cells. \=×1000
D. The strong (↑)
and weak (▲)
positive IAPP IR plasma cells. ×400
E. The negative
control section in which IAPP antiserum was replaced by PBS. (↑)
showing plasma cell. ×1000
Firstly,
the specificity of the above findings should be confirmed because
there was no IAPP expression in the plasma cells on the negative
control sections; and there were also IAPP-IR negative plasma cells
around the positive ones. Secondly, the significance of IAPP
expression in plasma cells should be studied. IAPP is mainly
secreted by islet B cells［7］.
Recent study indicated that besides regulating blood glucose, IAPP
could inhibit gastric acid secretion［4］,
and protect gastric mucosa［5］.
IAPP -IR cells of islet were markedly increased during the healing
process of rat gastric ulcer［8］.
The above-mentioned studies all suggested that IAPP is beneficial to
ulcer healing. As it is known, the plasma cells of gastric mucosa
come from B lymphocytes and they respond by synthesizing and
secreting IgA. It is observed, for the first time, that plasma cells
in gastric mucosa of peptic ulcer patients not only increased in the
number, but also expressed IAPP. Combined with our previous
observation that T and B lymphocytes of gastric mucosa increased in
peptic ulcer patients, it is reasonable to infer that some plasma
cells in gastric mucosa of peptic ulcer patients may transform to
ones expressing IAPP so as to maintain the high level of IAPP in the
gastric mucosa and help promote ulcer healing just as a growth
factor［9］.
CONCLUSION
It is found, for the first time, that IAPP was expressed in
plasma cells of gastric mucosa of peptic ulcer patients, which
provides morphologic evidence for the existence of neuro-endocrine-immune
network.
REFERENCES
1 Zheng ZT.Digestive Ulcer. Beijing: The
People＇s
Health Publis
hing House, 1998:306-325
2 Huang Y, Lu SJ, Dong JX, Li F. Changes of T and
B lymphocytes in gastric mucosa of peptic ulcer patients. Shijie
Huaren
Xiaohua
Zazhi, 1999:7:648
3 Ludvik B, Kautzky WA, Prager R, Thomaseth K,
Pacini G. Amylin: history and overview. Diabet Med,
1997;14(Suppl
2):S9-13
4 Clementi G, Caruso A, Cutuli VM, Prato A, de-Bernardis
E, Amico-Roxas M. Effect of amylin in various experimental
models of gastric ulcer. Eur J Pharmacol, 1997;332:209-213
5 Gedulin BR, Young AA. Hypoglycemia overrides
amylin-mediated regulation
of gastric emptying in rats. Diabetes,
1998;47:93-97
6 Rossowski WJ, Jiang NY, Coy DH. Adrenomedullin,
amylin, calcitonin gene-related peptide and their fragments are
potent
inhibitors of gastric acid secretion in rats. Eur J Pharmacol,
1997;336:51-63
7 Huang Y, Li ZC, Shi AR. Morphometric study on
coexistence of islet amyloid polypeptide with insulin in rat
pancreatic
islet
cells during postnatal development. Acta Anatomica Sinica,
1995:26:305-308
8 Li ZC, Shi AR. The morphometric study on IAPP-IR
cells and Ins-IR cells of islet during the healing process of
rat
experimental gastric ulcer. J Beijing Med Univ, 1995;27:333-335
9 Wookey PJ, Tikellis C, Nobes M, Casley D, Cooper
ME, Darby IA. Amylin as a growth factor during fetal and postnatal
development
of the rat kidney. Kidney Int, 1998;53:25-30
| |
PubMed
