Julia B Ewaschuk, Levinus A Dieleman, Centre of Excellence for Gastrointestinal Inflammation and Immunity Research, University of Alberta, Edmonton, Alberta, Canada
Correspondence to: Dr. Levinus A Dieleman, MD, PhD, Associate Professor of Medicine, 224 Zeidler Ledcore Building, 130 University Campus, University of Alberta, Edmonton, Alberta, T6G 2X8, Canada. firstname.lastname@example.org
Telephone: +1-780-4921888 Fax: +1-780-4921878
Received: 2006-05-20 Accepted: 2006-06-16
The prokaryotic and eukaryotic cells of the colon exist in a highly complex, but harmonious relationship. Disturbances in this remarkable symbiosis can result in the development of inflammatory bowel diseases (IBD). Although the etiology of IBD is not entirely understood, it is known that the chronic inflammation of Crohn’s disease, ulcerative colitis and chronic pouchitis are a result of an overly aggressive immune response to the commensal intestinal flora in genetically susceptible hosts. Recent studies have enhanced our ability to understand the interaction between the host and its intestinal microflora and the role the microflora plays in maintaining intestinal homeostasis. As we begin to understand the benefits conferred to the intestine by the microflora, the notion of modifying the composition of the bacterial load to improve human health has arisen. A significant body of research now exists investigating the role of probiotics and prebiotics in ameliorating chronic intestinal inflammation. This article will begin with an overview of the role of the commensal microflora in maintaining mucosal immune homeostasis, and how a dysregulated immune response to the intestinal microflora results in IBD. This will be followed by a summary of the use of probiotics and prebiotics in experimental and human IBD.
© 2006 The WJG Press. All rights reserved.
Key words: Colitis; Crohn’s disease; Microflora; Immu-nity; Probiotics; Prebiotics
Ewaschuk JB, Dieleman LA. Probiotics and prebiotics in chronic inflammatory bowel diseases. World J Gastroenterol 2006; 12(37): 5941-5950
At birth, the
gastrointestinal tract is a sterile environment. Initial exposure of the
gut to microbes occurs during the birthing process from the maternal
fecal and vaginal flora. Within a few months after birth, a relatively
stable microbial population is established. This abundant,
diverse and dynamic intestinal microflora normally lives in a complex,
symbiotic relationship with the eukaryotic cells of the mucosa. About
100 trillion bacterial cells benefit from the constant nutrient flow,
stable temperature and niches for various metabolic requirements
provided by the intestinal environment. Likewise, the host benefits from
the ability of the intestinal microflora to synthesize vitamin K, exert
trophic effects on intestinal epithelial cells, salvage energy from
unabsorbed food by producing short-chain fatty acids, inhibit the growth
of pathogens, sustain intestinal barrier integrity and maintain mucosal
immune homeostasis. Studies from germ-free animals reveal that the
absence of resident intestinal microflora results in significant
alterations in intestinal structure and function, including slender
villi, shallow crypts, low leukocyte count[2,3], a decrease
in the number and density of Peyer’s
In their co-evolution with bacteria, vertebrates develop pattern-recognition receptors, which are activated by specific molecular patterns unique to bacteria, fungi and viruses that are absent in eukaryotes (lipopolysaccharides, peptidoglycan, ssRNA, muramyl dipeptide, flagellins, etc). These include the Toll-like receptors (TLRs) and nucleotide oligomerization domains (NODs). TLRs and NODs are critical for the initiation of innate immune defense responses. Activation of their signaling cascades usually results in the production of pro-inflammatory cytokines. TLR signaling also provides a link between innate and adaptive immunity, as TLR signaling results in the maturation of dendritic cells, which activate adaptive immune responses. Although stimulation of these receptors in most parts of the immune system results in production of inflammatory cytokines, these ligands are not only tolerated by the gut mucosal immune system, but also essential for adaptation to intestinal bacteria and maintenance of homeostasis. The tolerance to the intestinal microflora is not completely understood, but several aspects of commensal physiology have been defined which contribute to their inability to activate the immune system. Some commensal bacteria can modify TLR ligands, resulting in a hypoactive immune response. For example, the endotoxic portion of LPS is pentacylated in many Bacteroides species, and has minimal toxicity. An important feature of commensal bacteria is their inability to penetrate the intestinal epithelial barrier. If some of these organisms do penetrate, they are usually rapidly phagocytosed by the innate mucosal immune system. Indeed, in a healthy host, the systemic immune system appears to be ignorant of the intestinal microflora. Maintaining tolerance to these intestinal bacteria is a remarkable accomplishment achieved by the mucosal immune system, and disturbances in this bacterial-epithelial homeostasis result in considerable deleterious effects on the host.
Role of the commensal flora in IBD
Although many studies have investigated the possibility of a single infectious agent causing Crohn’s disease and ulcerative colitis, also called chronic inflammatory bowel disease (IBD), none has yet been discovered. The intestinal bacteria are now believed to be involved in the initiation and perpetuation of IBD. The prevailing theory explaining the development of IBD is that the adaptive immune system is hyper-responsive to the commensal intestinal microflora in genetically susceptible individuals. This hypothesis is supported by several observations: most inflammation occurs in areas with the highest density of intestinal bacteria, broad spectrum antibiotics improve chronic intestinal inflammation, and surgical diversion of the fecal stream can prevent recurrence of Crohn’s disease. Most importantly, despite differences in the pathogenesis of chronic intestinal inflammation, a consistent feature of many animal models of IBD (such as IL-10 knockout mice and HLA-B27 transgenic rats) is the failure to develop chronic intestinal inflammation when these animals are raised in germ-free conditions[11,12]. Dysbiosis is also observed in IBD patients. Adherent and intramucosal bacteria, particularly Bacteroides spp, Escherichia coli (E. coli) and Enterobacterium spp are more abundant in patients with Crohn’s disease (CD) than in controls[13,14] (Figure 1). Bacterial overgrowth and dysbiosis are also associated with the development of chronic pouchitis, the inflammation of the ileal reservoir created after ileo-anal anastamosis following colectomy in ulcerative colitis (UC) patients. In addition, several selected commensal bacterial species can induce and perpetuate colitis in genetically susceptible rodent models of chronic intestinal inflammation[16,17] (Figure 1).
Other immune dysfunctions in IBD include aberrant secretion of pro-inflammatory cytokines and chemokines by intestinal epithelial cells and mucosal immune cells[18-20], altered TLR4 signaling, defective antigen presenting cell function and lack of T-cell apoptosis. It is now clear that ignorance of the systemic immune system to commensal intestinal microflora is lost in IBD patients, as shown by enhanced and persistent cell-mediated and humoral immune responses to these bacteria.
At least 7 genetic loci conferring susceptibility to CD, ulcerative colitis (UC) or both, have been identified. Interestingly, such susceptibility genes associated with CD involve polymorphisms of the NOD2 gene (the pattern recognition receptor for muramyl dipeptide) which can result in hampered innate immune functions by impairment of TLR function, defective clearance of invasive bacteria by macrophages, and decreased production of defensins.
The recognition of the compelling association between intestinal microflora and the development of IBD has led to an abundance of studies investigating the therapeutic potential of altering luminal bacteria using probiotics and/or prebiotics.
Probiotics, prebiotics and synbiotics
Probiotics are defined as living organisms in food and dietary supplements which, upon ingestion, improve the health of the host beyond their inherent basic nutrition. Probiotics are typically lactic acid bacteria selected from the gut flora, and are able to survive stomach acid and bile, maintain viability throughout extended periods of storage and are safe for human consumption. Other species have also shown some beneficial effects, such as E. coli Nissle 1917 and Saccharomyces boulardii (Figure 1). Probiotic bacteria have verifiable beneficial properties, including the ability to improve epithelial barrier function, modulate the mucosal immune system, and alter the intestinal flora.
Prebiotics are non-digestible dietary carbohydrates, e.g., lactosucrose, fructo- and galacto-oligosaccharides, inulin, psyllium, bran, germinated barley (Figure 2), which stimulate the growth and metabolism of endogenous enteric protective bacteria upon consumption. Beneficial effects of prebiotics are also associated with changes in colonic short-chain fatty acids (SCFA) due to fermentation by colonic bacteria. Synbiotics are combinations of probiotics and prebiotics, and are also an emerging therapeutic modality. Restoration of normal microflora using probiotics, prebiotics or synbiotics has been investigated in numerous gastrointestinal and other disease states, including infectious diarrhea, H pylori infection, irritable bowel syndrome, colorectal cancer, lactase deficiency, pancreatitis, atopy, and IBD.
Protective mechanisms of probiotics by ameliorating chronic intestinal inflammation
Probiotic bacteria have beneficial effects on the intestinal epithelia both directly and indirectly, including enhanced barrier function, modulation of the mucosal immune system, production of antimicrobials, and alteration of the intestinal microflora (Figure 3).
Alteration of the mucosal immune system. The presence of probiotics has been shown to result in several modifications in the mucosal immune response, including augmented antibody production[32,33], increased phagocyte and natural killer cell activity[35-38], modulation of the nuclear factor kappa-B (NFkB) pathway[39-41], and induction of T cell apoptosis. Generally, probiotics increase the production of intestinal anti-inflammatory cytokines (such as IL-10 and TGF-b), while reducing the production of pro-inflammatory cytokines (e.g., TNF-a, interferon-g, IL-8)[43-46]. Several probiotic bacteria, including B. breve, Streptococcus thermophilus, B. bifidum and Ruminococcus gnavus have been shown to secrete metabolites that reduce LPS-induced TNF-a secretion. L. reuteri reduces TNF-a and Salmonella typhimurium induceds IL-8 secretion in vitro, by inhibiting nuclear translocation of NFkB and preventing the degradation of ΙkB. Administration of the probiotic cocktail VSL#3 (consisting of L. acidophilus, L. bulgaricus, L. casei, L, plantarum, B. breve, B. infantis, B. longum, S. thermophilus) to IL-10 deficient mice results in colitis reduction and a concomitant reduction in mucosal secretion of TNF-a and interferon-g. E. coli Nissle 1917 is able to down-regulate the expansion of newly recruited T-cells into the mucosa and limit chronic intestinal inflammation. In SAMP1/Yit mice, Lactobacillus casei strain Shirota inhibits IL-6 production in LPS-stimulated large intestinal lamina propria mononuclear cells and down-regulates nuclear translocation of NFkB. Patients with a recent ileo-anal pouch anastamosis who responded to probiotic therapy have reduced mRNA levels of IL-1b, IL-8 and IFN-g, and fewer polymorphonuclear cells compared with patients who receive placebo. Probiotic treatment has also been shown to reduce IFN-g and IL1-a expression and decrease inducible-nitric oxide synthase and gelatinase activities in pouch biopsy samples from patients with pouchitis. In mucosal explants of ileal specimens from patients with Crohn’s disease, probiotics reduced TNF-a release and the number of CD4 cells. In addition to live probiotics, components of probiotic bacteria can also exert effects on the mucosal immune system. For example, genomic DNA isolated from VSL#3 inhibits TNF-a-induced IL-8 secretion, mitogen-activated protein kinase activation and NFkB activation in HT-29 cells.
Improved barrier function. Various probiotic bacteria can enhance intestinal epithelial barrier function. For example, oral administration of VSL#3 results in normalization of impaired colonic barrier function and restoration of intestinal epithelial integrity in IL-10 deficient mice and enhancement of epithelial resistance in T-84 cells. Barrier function was enhanced not only by live bacteria, but also by a proteinaceous secreted product of VSL#3. Several strains of lactobacilli are also capable of up-regulating intestinal MUC3 mRNA expression, thereby improving barrier function by increasing the mucus layer[55,56]. Lactobacillus GG (L. GG) improves barrier function by inhibiting apoptosis of intestinal epithelial cells. S. thermophilus and L. acidophilus have been shown to enhance phosphorylation of actinin and occludin in the tight junction, thereby preventing the invasion of enteroinvasive E. coli into human intestinal epithelial cells.
Alteration of the intestinal flora. Probiotics suppress the growth and invasion of pathogens in several ways. They competitively exclude pathogenic bacteria by occupying the limited physical space in the mucus layer and on epithelial cells. They also engage pattern-recognition receptors and consume substrate otherwise available to other (pathogenic) microbes. In addition, probiotics render their microenvironment inauspicious for pathogens by secreting antimicrobial substances such as hydrogen peroxide, organic acids, and bacteriocins. For example, both in vitro and in vivo experiments demonstrate that B. infantis suppresses the growth of Bacteroides vulgatus. VSL#3 has been shown to inhibit Salmonella dublin invasion into T-84 cells. Patients with pouchitis treated with VSL#3 have been demonstrated to have increased bacterial diversity in the pouch, and decreased fungal diversity.
Probiotics may also alter the intestinal microflora by changing the fatty acid profile in the colon. VSL#3 probiotic strains are also capable of converting linoleic acid to conjugated linoleic acid, a fatty acid with anti-inflammatory and anti-carcinogenic properties.
Use of probiotics in inflammatory bowel disease treatment
Results from various animal studies and clinical trials using probiotics to treat intestinal inflammation have generated considerable excitement. Data are now emerging which suggest that probiotics are capable of preventing relapse of chronic intestinal inflammation. Some probiotics can even treat mild to moderately active IBD[62,63]. However, at present there is a relative lack of rigorously designed, randomized, placebo-controlled trials. Level 1 evidence is only available for the use of probiotics in post-operative chronic pouchitis while level 2 and 3 evidence supports the use of probiotics in treatment of CD and UC (Table 1).
More than 20 animal models of IBD are available and have been widely used to study the efficacy and mechanisms of probiotics in ameliorating inflammation in order to provide support for human clinical trials. In IL-10 knockout mice, L. plantarum 299v, L. reuteri, L. salivarius subspecies salivarius 433118, B. infantis 35624, L. salivarius subspecies salivarius UCC118 and VSL#3 have all been shown to successfully attenuate intestinal inflammation. L. GG prevents recurrent colitis in HLA-B27 transgenic rats after antibiotic treatment, whereas L. plantarum has no effect. Both VSL#3 and L. GG significantly ameliorate sulfhydryl-blocker iodoacetamide-induced colitis in rats, whereas they have no effect on dinitrobenzene sulfonic acid-induced colitis. Improved inflammation in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis has also been demonstrated after oral administration of L. salivarius ssp. salivarius CECT5713 and L. plantarum NCIMB8826[70,71]. Dextran sulphate sodium (DSS)-induced colitis in mice is ameliorated by soluble bacterial antigens extracted from E. coli (strain Laves) or by Bifidobacterium strains breve, catenulatum, and longum[72,73]. Daily administration of live but not heat-killed auto-aggregating L. crispatus reduces the severity of DSS-colitis in mice. L. reuteri significantly reduces the colonic inflammation caused by both acetic acid and methotrexate in rats[75,76]. Interestingly, DNA from VSL#3 has been reported to reduce colonic inflammation, thus improving intestinal barrier function in IL-10 KO mice and DSS-induced colitis[41,77].
Probiotics can maintain antibiotic-induced remission in patients with chronic pouchitis after colectomy for refractory UC. Gionchetti et al have completed placebo-controlled trials using the probiotic cocktail VSL#3 in patients with chronic relapsing pouchitis, showing that the remission rate after 1 year is 90% in the group treated with VSL3# versus 60% in the placebo group. Another study by the same group also showed that VSL#3 is also capable of preventing the development of chronic pouchitis during the first year after pouch surgery for UC. Ten percent of patients who took VSL#3 developed pouchitis, compared with 40% of the placebo group. A double-blinded, prospective, randomized placebo-controlled trial was carried out in 20 patients treated with L.GG versus placebo for 3-mo. In contrast to the study with the probiotic cocktail VSL#3, no significant differences were observed in chronic pouchitis disease activity in L.GG-treated patients despite an increased total fecal lactobacilli:anaerobe ratio, illustrating the inefficacy of L. GG for this condition.
Numerous studies have investigated the use of probiotics for maintenance of remission of UC in humans. A small study investigating the use of non-pathogenic E. coli Nissle 1917 versus low-dose mesalamine showed that it can maintain remission of quiescent UC, with a relapse rate of 16%-67% in those treated with E.coli versus 11%-73% in the mesalamine group. In a double-blind, randomized trial involving 327 patients, Kruis et al compared the effectiveness of an oral preparation of E. coli Nissle 1917 with mesalamine for maintaining remission of UC, and found that at the end of the 12-mo study, there is no significant difference between the two study groups, with relapses occurring in 36.4% of the E. coli Nissle 1917 group and 33.9% of the mesalamine group (significant equivalence, P = 0.003). Recently, Zocco et al investigated the efficacy of L. GG in maintaining remission of UC and found that there is no difference in relapse rate between the 3 groups after 6 and 12 mo. However, L. GG is more effective than standard mesalamine treatment in prolonging relapse-free time (P < 0.05). An open-label study of VSL#3 showed that 15/20 UC patients remain in remission after 1 year.
Several studies have addressed treatment of established UC with probiotic therapy. A study using E. coli Nissle 1917 has demonstrated its equivalence to mesalamine for inducing remission of UC. Ishikawa et al evaluated Bifidobacterium-fermented milk in the treatment of UC, and symptoms of exacerbation were observed in 3 of 11 patients in the treated group versus 9 of 10 patients in the untreated group after 1 year (P = 0.01). However, no difference was observed in endoscopic disease activity. A placebo-controlled trial with bifidobacteria-fermented milk for 12 wk in UC patients with active disease showed that endoscopic disease activity index and histological score are significantly reduced in the treatment group compared with those in placebo group. Uncontrolled administration of daily VSL#3 for 3 mo induces remission in 19 of 30 patients (63%), with a response rate of 87%. As in all of these reported studies, the increased luminal probiotic bacteria return to baseline levels within one month after stopping probiotic treatment, indicating only transient colonization by these probiotic bacteria. In another small study administration of a combination of 3 bifidobacterium species for 2 mo was superior (20% remission) to placebo (93% remission) in maintaining remission of UC induced by sulfasalazine and glucocorticoids. This effect correlates with decreased mucosal TNF-a, IL-1b and increased mucosal IL-10 levels. An interesting study by Borody et al showed that altering the gut microflora in UC patients achieved dramatic outcomes by administration of a freshly prepares enema from a healthy donor to six patients with relapsing refractory UC after broad spectrum antibiotics. This results in a remarkable reversal of all symptoms after 4 mo and sustained remission after 1-3 year of treatment.
Thus far, the use of probiotics for the prevention and treatment of CD is less substantiated than for the prevention and treatment of UC, although some studies certainly show promise. The effect of probiotics in maintaining remission of CD has been reported in an open-label study in patients receiving mesalamine alone versus mesalamine and S. boulardii. At 6 mo, 37.5% of patients had a clinical relapse in the former group versus 6.3% of patients in the probiotic group. In an open-labeled study, McCarthy et al reported that oral administration of L. salivarius UCC118 significantly reduces disease activity in patients with mild to moderate CD. In a randomized, placebo-controlled pilot study, patients with CD were treated with steroids and randomized to non-pathogenic E. coli Nissle 1917 as probiotic therapy or placebo. After 1 year, there were fewer relapses in the probiotic group, but this was not statistically significant. Despite these promising studies, there are numerous reports on the inefficacy of some probiotics in CD. A randomized, placebo-controlled study of 98 patients showed that L. johnsonii LA1 is ineffective in preventing postoperative recurrence of CD. A placebo-controlled trial with L. GG is also ineffective in preventing post-operative recurrence of CD in patients undergoing bowel resection. Other studies have failed to detect benefits of L. GG in maintaining remission of CD in children and adults[97,98]. Although L. GG has demonstrated its efficacy in treating rotaviral and antibiotic-associated diarrhea, results in IBD patients have been particularly underwhelming for this bacterial species, highlighting the species-specifity of colitis protection by probiotics.
Prebiotics in inflammatory bowel disease
Studies using prebiotics have been performed mostly in animal models. Lactulose and inulin have been shown to attenuate inflammation in IL-10 knockout mice and DSS-induced colitis respectively[46,101]. The combination of inulin and oligofructose (mixture 1:1) is also effective in preventing the development of colitis in HLA-B27 transgenic rats. This beneficial effect is observed in conjunction with an increase of intestinal bifidobacteria and lactobacilli. Another study in HLA-B27 transgenic rats showed that the effects of the synbiotic “SIM”, a combination of lactobacilli, bifidobacteria and the prebiotic inulin, are attributed to the inulin rather than the probiotics. The ingested probiotic bacteria are not detectable in the cecal content, yet the microflora profile of their cecal contents is altered. In that study, inulin was also shown to specifically stimulate the growth of Bifidobacterium animalis. DSS-induced colitis rats fed with goat’s milk oligosaccharides maintain their body weight, have reduced colonic myeloperoxidase activity and clinical symptoms and increased MUC-3 expression compared with control rats. Goat’s milk oligosaccharides also causes decreased anorexia, weight loss, bowel wall thickening and necrotic lesions in TNBS-induced colitis in rats, compared with untreated controls. In another study of TNBS-induced colitis in rats, a 2-wk feeding of lactulose prior to the induction of colitis reduces myeloperoxidase activity, colonic TNFa and leukotriene B production, in conjunction with an increase of lactobacilli and bifidobacteria species in feces. Lactulose has also demonstrated a dose-dependent beneficial effect on DSS-induced colitis in rats, including improvements of colonic ulceration areas, body weight changes, diarrhea, bloody stools and a reduction of myeloperoxidase activity and microscopic colitis.
However, not all studies using prebiotics have resulted in positive outcomes. Moreau et al found that fructo-oligosaccharides are ineffective in improving DSS-induced colitis in rats. Holma et al have reported a similar inefficacy of galacto-oligosaccharides in TNBS-colitis rats.
Several studies have investigated the use of an insoluble mixture of glutamine-rich protein and hemicellulose-rich dietary fiber termed germinated barley foodstuff (GBF). Fukuda et al found that feeding GBF to rats with DSS-induced colitis resultes in significantly reduced colonic inflammation scores, and increased butyrate concentrations in cecal contents. Kanauchi et al have observed similar results in DSS-colitis rats, and further determined that the dietary fiber component rather than the protein component of the GBF is responsible for the beneficial effects of GBF.
Table 2 lists the clinical trials using prebiotics to treat IBD. Although there is a paucity of human studies
using prebiotics, the few emerging studies showed that there is
potential for this treatment modality. A multi-centered open-label trial
reported that oral administration of GBF to patients with mild to
moderately active UC for 24 wk resultes in a significant decrease in
clinical activity index, compared to controls. An
open-label study of 22 UC patients in remission showed that a daily oral
intake of 20 g GBF resultes in a significantly improved clinical
activity index and endoscopic score at 3, 6 and 12 mo, and a reduced
relapse rate, compared with controls. A recent
randomized, double-blinded controlled trial by Furrie
A small, uncontrolled study of 15 active CD patients reported that 21 d of fructo-oligosaccharide (15 g) intake results in a significant decrease of disease activity, an increase of intestinal bifidobacteria and modifications of Toll-like receptors and IL-10 expression in mucosal dendritic cells.
The link between intestinal microflora and IBD is now well established, and altering the composition of the microflora using probiotics and prebiotics holds promise as a therapeutic strategy for ameliorating chronic intestinal inflammation. Future developments in this field must include rigorous double-blind, placebo-controlled trials, using probiotics and prebiotics along with a further understanding of their protective mechanisms. Due to their excellent safety profile and lack of serious side effects, there are few contraindications to the consumption of prebiotics, probiotics and synbiotics by IBD patients. Further understanding of the interactions between microbes and gastrointestinal tract will help identify which strains of bacteria and/or which prebiotics may be effective in the treatment of different types of chronic inflammatory disease.
1 Fanaro S, Chierici R,
Guerrini P, Vigi V. Intestinal microflora in early infancy: composition
and development. Acta
2 Sharma R, Schumacher U,
Ronaasen V, Coates M. Rat intestinal mucosal responses to a microbial
flora and different
3 Szentkuti L, Riedesel H, Enss
ML, Gaertner K, Von Engelhardt W. Pre-epithelial mucus layer in the
colon of conventional
4 Maeda Y, Noda S, Tanaka K,
Sawamura S, Aiba Y, Ishikawa H, Hasegawa H, Kawabe N, Miyasaka M, Koga
5 Husebye E, Hellstrom PM,
Sundler F, Chen J, Midtvedt T. Influence of microbial species on small
6 Medzhitov R. Toll-like receptors and innate immunity. Nat Rev Immunol 2001; 1: 135-145 PubMed
7 Sansonetti PJ. War and peace at mucosal surfaces. Nat Rev Immunol 2004; 4: 953-964 PubMed
8 Williamson SI, Wannemuehler
MJ, Jirillo E, Pritchard DG, Michalek SM, McGhee JR. LPS regulation of
9 Macpherson AJ,
Gatto D, Sainsbury E, Harriman GR, Hengartner H, Zinkernagel RM. A
primitive T cell-independent
10 Bamias G,
Nyce MR, De La Rue SA, Cominelli F. New concepts in the pathophysiology
of inflammatory bowel disease.
11 Sellon RK,
Tonkonogy S, Schultz M, Dieleman LA, Grenther W, Balish E, Rennick DM,
Sartor RB. Resident enteric
12 Taurog JD,
Richardson JA, Croft JT, Simmons WA, Zhou M, Fernandez-Sueiro JL, Balish
E, Hammer RE. The germfree
13 Swidsinski A,
Ladhoff A, Pernthaler A, Swidsinski S, Loening-Baucke V, Ortner M, Weber
J, Hoffmann U, Schreiber S,
14 Seksik P,
Rigottier-Gois L, Gramet G, Sutren M, Pochart P, Marteau P, Jian R, Dore
J. Alterations of the dominant faecal
Embden JG, Schouten WR, van Lieshout LM. Pouchitis: result of
microbial imbalance? Gut 1994; 35: 658-
16 Sartor RB.
Probiotic therapy of intestinal inflammation and infections. Curr
Opin Gastroenterol 2005; 21: 44-50
17 Sydora B,
Lupicki M, Martin SM, Backer J, Churchill TA, Fedorak RN, Dieleman LA.
Enterobacter Cloacae Induces early
18 Stadnyk AW.
Intestinal epithelial cells as a source of inflammatory cytokines and
chemokines. Can J Gastroenterol
19 Kwon JH,
Keates S, Bassani L, Mayer LF, Keates AC. Colonic epithelial cells are a
major site of macrophage
20 Dieleman LA,
Hoentjen F, Qian BF, Sprengers D, Tjwa E, Torres MF, Torrice CD, Sartor
RB, Tonkonogy SL. Reduced
21 Cario E,
Rosenberg IM, Brandwein SL, Beck PL, Reinecker HC, Podolsky DK.
Lipopolysaccharide activates distinct
22 Qian BF,
Tonkonogy SL, Hoentjen F, Dieleman LA, Sartor RB. Dysregulated luminal
bacterial antigen-specific T-cell
23 Boirivant M,
Marini M, Di Felice G, Pronio AM, Montesani C, Tersigni R, Strober W.
Lamina propria T cells in Crohn’s
24 Duchmann R,
Kaiser I, Hermann E, Mayet W, Ewe K, Meyer zum Buschenfelde KH.
Tolerance exists towards resident
25 Ahmad T,
Satsangi J, McGovern D, Bunce M, Jewell DP.
Review article: the genetics of inflammatory bowel disease.
26 Hugot JP,
Chamaillard M, Zouali H, Lesage S, Cezard JP, Belaiche J, Almer S, Tysk
C, O’Morain CA, Gassull M, Binder V,
27 Netea MG,
Kullberg BJ, de Jong DJ, Franke B, Sprong T, Naber TH, Drenth JP, Van
der Meer JW. NOD2 mediates anti-
28 Kobayashi KS,
Chamaillard M, Ogura Y, Henegariu O, Inohara N, Nunez G, Flavell RA.
Nod2-dependent regulation of
29 Wehkamp J,
Harder J, Weichenthal M, Schwab M, Schaffeler E, Schlee M, Herrlinger
KR, Stallmach A, Noack F, Fritz P,
30 Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989; 66: 365-378 PubMed
31 Cherbut C. Inulin and oligofructose in the dietary fibre concept. Br J Nutr 2002; 87 Suppl 2: S159-S162 PubMed
32 Rinne M,
Kalliomaki M, Arvilommi H, Salminen S, Isolauri E. Effect of probiotics
and breastfeeding on the
33 Kaila M,
Isolauri E, Soppi E, Virtanen E, Laine S, Arvilommi H. Enhancement of
the circulating antibody secreting cell
34 Shu Q, Gill
HS. Immune protection mediated by the probiotic Lactobacillus rhamnosus
HN001 (DR20) against Escherichia
35 Gill HS,
Rutherfurd KJ, Cross ML, Gopal PK. Enhancement of immunity in the
elderly by dietary supplementation with the
36 Gill HS,
Rutherfurd KJ, Cross ML. Dietary probiotic supplementation enhances
natural killer cell activity in the elderly: an
37 Sheih YH,
Chiang BL, Wang LH, Liao CK, Gill HS. Systemic immunity-enhancing
effects in healthy subjects following
38 Ogawa T,
Asai Y, Tamai R, Makimura Y, Sakamoto H, Hashikawa S, Yasuda K. Natural
killer cell activities of synbiotic
39 Petrof EO,
Kojima K, Ropeleski MJ, Musch MW, Tao Y, De Simone C, Chang EB.
Probiotics inhibit nuclear factor-kappaB
40 Tien MT,
Girardin SE, Regnault B, Le Bourhis L, Dillies MA, Coppee JY,
Bourdet-Sicard R, Sansonetti PJ, Pedron T. Anti-
41 Jijon H,
Backer J, Diaz H, Yeung H, Thiel D, McKaigney C, De Simone C, Madsen K.
DNA from probiotic bacteria
42 Di Marzio L,
Russo FP, D’Alo S, Biordi L, Ulisse S, Amicosante G, De Simone C, Cifone
MG. Apoptotic effects of selected
43 Haller D,
Bode C, Hammes WP, Pfeifer AM, Schiffrin EJ, Blum S. Non-pathogenic
bacteria elicit a differential cytokine
44 Maassen CB,
van Holten-Neelen C, Balk F, den Bak-Glashouwer MJ, Leer RJ, Laman JD,
Boersma WJ, Claassen E.
45 Morita H,
He F, Fuse T, Ouwehand AC, Hashimoto H, Hosoda M, Mizumachi K, Kurisaki
J. Adhesion of lactic acid
46 Madsen KL,
Doyle JS, Jewell LD, Tavernini MM, Fedorak RN. Lactobacillus species
prevents colitis in interleukin 10 gene-
47 Menard S,
Candalh C, Bambou JC, Terpend K, Cerf-Bensussan N, Heyman M. Lactic acid
bacteria secrete metabolites
48 Ma D,
Forsythe P, Bienenstock J. Live Lactobacillus reuteri is essential for
the inhibitory effect on tumor necrosis factor
49 Madsen K,
Cornish A, Soper P, McKaigney C, Jijon H, Yachimec C, Doyle J, Jewell L,
De Simone C. Probiotic bacteria
50 Sturm A,
Rilling K, Baumgart DC, Gargas K, Abou-Ghazale T, Raupach B, Eckert J,
Schumann RR, Enders C, Sonnenborn
51 Matsumoto S,
Hara T, Hori T, Mitsuyama K, Nagaoka M, Tomiyasu N, Suzuki A, Sata M.
52 Lammers KM,
Vergopoulos A, Babel N, Gionchetti P, Rizzello F, Morselli C, Caramelli
E, Fiorentino M, d’Errico A, Volk
53 Ulisse S,
Gionchetti P, D’Alo S, Russo FP, Pesce I, Ricci G, Rizzello F, Helwig U,
Cifone MG, Campieri M, De Simone C.
54 Borruel N,
Carol M, Casellas F, Antolin M, de Lara F, Espin E, Naval J, Guarner F,
Malagelada JR. Increased mucosal
55 Mack DR,
Michail S, Wei S, McDougall L, Hollingsworth MA. Probiotics inhibit
enteropathogenic E. coli adherence in vitro
56 Mack DR,
Ahrne S, Hyde L, Wei S, Hollingsworth MA. Extracellular MUC3 mucin
secretion follows adherence of
57 Yan F, Polk
DB. Probiotic bacterium prevents cytokine-induced apoptosis in
intestinal epithelial cells. J Biol Chem 2002;
58 Resta-Lenert S,
Barrett KE. Live probiotics protect intestinal epithelial cells from the
effects of infection with
59 Shiba T, Aiba Y, Ishikawa H,
Ushiyama A, Takagi A, Mine T, Koga Y. The suppressive effect of
60 Kuhbacher T,
Ott SJ, Helwig U, Mimura T, Rizzello F, Kleessen B, Gionchetti P, Blaut
M, Campieri M, Folsch UR, Kamm
61 Ewaschuk JB,
Walker JW, Diaz H, Madsen KL. Bioproduction of conjugated linoleic acid
by probiotic bacteria occurs in
62 Fedorak RN,
Madsen KL. Probiotics and the management of inflammatory bowel disease.
Inflamm Bowel Dis 2004; 10:
63 Sartor RB.
Therapeutic manipulation of the enteric microflora in inflammatory bowel
diseases: antibiotics, probiotics,
64 Jurjus AR,
Khoury NN, Reimund JM. Animal models of
inflammatory bowel disease. J Pharmacol Toxicol Methods 2004;
65 Schultz M,
Veltkamp C, Dieleman LA, Grenther WB, Wyrick PB, Tonkonogy SL, Sartor RB.
Lactobacillus plantarum 299V
66 McCarthy J,
O’Mahony L, O’Callaghan L, Sheil B, Vaughan EE, Fitzsimons N, Fitzgibbon
J, O’Sullivan GC, Kiely B, Collins
67 O’Mahony L,
Feeney M, O’Halloran S, Murphy L, Kiely B, Fitzgibbon J, Lee G,
O’Sullivan G, Shanahan F, Collins JK.
68 Dieleman LA,
Goerres MS, Arends A, Sprengers D, Torrice C, Hoentjen F, Grenther WB,
Sartor RB. Lactobacillus GG
69 Shibolet O, Karmeli F, Eliakim
R, Swennen E, Brigidi P, Gionchetti P, Campieri M, Morgenstern S,
70 Peran L, Camuesco D, Comalada
M, Nieto A, Concha A, Diaz-Ropero MP, Olivares M, Xaus J, Zarzuelo A,
71 Pavan S, Desreumaux P,
Mercenier A. Use of mouse models to evaluate the persistence, safety,
72 Konrad A, Mahler M, Flogerzi
B, Kalousek MB, Lange J, Varga L, Seibold F. Amelioration of murine
colitis by feeding a
73 Setoyama H, Imaoka A, Ishikawa
H, Umesaki Y. Prevention of gut inflammation by Bifidobacterium in
74 Castagliuolo I, Galeazzi F,
Ferrari S, Elli M, Brun P, Cavaggioni A, Tormen D, Sturniolo GC, Morelli
L, Palu G. Beneficial
75 Fabia R, Ar’Rajab A, Johansson
ML, Willen R, Andersson R, Molin G, Bengmark S. The effect of exogenous
76 Mao Y, Nobaek S, Kasravi B,
Adawi D, Stenram U, Molin G, Jeppsson B. The effects of Lactobacillus
strains and oat fiber
77 Rachmilewitz D, Katakura K,
Karmeli F, Hayashi T, Reinus C, Rudensky B, Akira S, Takeda K, Lee J,
Takabayashi K, Raz
78 Gionchetti P, Rizzello F,
Venturi A, Brigidi P, Matteuzzi D, Bazzocchi G, Poggioli G, Miglioli M,
Campieri M. Oral
79 Gionchetti P, Rizzello F,
Helwig U, Venturi A, Lammers KM, Brigidi P, Vitali B, Poggioli G,
Miglioli M, Campieri M.
80 Kuisma J, Mentula S, Jarvinen
H, Kahri A, Saxelin M, Farkkila M. Effect of Lactobacillus rhamnosus GG
on ileal pouch
81 Kruis W, Schutz E, Fric P,
Fixa B, Judmaier G, Stolte M. Double-blind comparison of an oral
Escherichia coli preparation
82 Kruis W, Fric P, Pokrotnieks
J, Lukas M, Fixa B, Kascak M, Kamm MA, Weismueller J, Beglinger C,
Stolte M, Wolff C,
83 Zocco MA, dal Verme LZ,
Cremonini F, Piscaglia AC, Nista EC, Candelli M, Novi M, Rigante D,
Cazzato IA, Ojetti V,
84 Venturi A, Gionchetti P,
Rizzello F, Johansson R, Zucconi E, Brigidi P, Matteuzzi D, Campieri M.
Impact on the
85 Rembacken BJ, Snelling AM,
Hawkey PM, Chalmers DM, Axon AT. Non-pathogenic Escherichia coli versus
86 Ishikawa H, Akedo I, Umesaki
Y, Tanaka R, Imaoka A, Otani T. Randomized controlled trial of the
87 Kato K, Mizuno S, Umesaki Y,
Ishii Y, Sugitani M, Imaoka A, Otsuka M, Hasunuma O, Kurihara R, Iwasaki
A, Arakawa Y.
88 Bibiloni R, Fedorak RN,
Tannock GW, Madsen KL, Gionchetti P, Campieri M, De Simone C, Sartor RB.
89 Cui HH,
Chen CL, Wang JD, Yang YJ, Cun Y, Wu JB, Liu YH, Dan HL, Jian YT, Chen
XQ. Effects of probiotic on intestinal
90 Borody TJ,
Warren EF, Leis S, Surace R, Ashman O. Treatment of ulcerative colitis
using fecal bacteriotherapy. J Clin
91 Guslandi M,
Mezzi G, Sorghi M, Testoni PA. Saccharomyces
boulardii in maintenance treatment of Crohn‘s disease. Dig
92 McCarthy J,
O’Mahony L, Dunne C, Kelly P, Feeney M, Kiely B, O’Sullivan G, Collins
JK, Shanahan F. An open trial of a
93 Malchow HA.
Crohn’s disease and Escherichia coli. A new approach in therapy to
maintain remission of colonic Crohn’s
94 Marteau P,
Lemann M, Seksik P, Laharie D, Colombel JF, Bouhnik Y, Cadiot G, Soule
JC, Bourreille A, Metman E,
95 Prantera C,
Scribano ML, Falasco G, Andreoli A, Luzi C. Ineffectiveness of
probiotics in preventing recurrence after
96 Bousvaros A,
Guandalini S, Baldassano RN, Botelho C, Evans J, Ferry GD, Goldin B,
Hartigan L, Kugathasan S, Levy J,
97 Schultz M,
Timmer A, Herfarth HH, Sartor RB, Vanderhoof JA, Rath HC. Lactobacillus
GG in inducing and maintaining
98 Prantera C, Scribano ML. Probiotics and Crohn’s disease. Dig Liver Dis 2002; 34 Suppl 2: S66-S67 PubMed
99 Szymanski H,
Pejcz J, Jawien M, Chmielarczyk A, Strus M, Heczko PB.
Treatment of acute infectious diarrhoea in infants
100 Vanderhoof JA,
Whitney DB, Antonson DL, Hanner TL, Lupo JV, Young RJ. Lactobacillus GG
in the prevention of
101 Videla S,
Vilaseca J, Antolin M, Garcia-Lafuente A, Guarner F, Crespo E, Casalots
J, Salas A, Malagelada JR. Dietary
102 Hoentjen F,
Welling GW, Harmsen HJ, Zhang X, Snart J, Tannock GW, Lien K, Churchill
TA, Lupicki M, Dieleman LA.
103 Schultz M,
Munro K, Tannock GW, Melchner I, Gottl C, Schwietz H, Scholmerich J,
Rath HC. Effects of feeding a probiotic
F, Debras E, Nieto A, Concha A, Galvez J, Lopez-Huertas E, Boza J,
Obled C, Xaus J. Oligosaccharides
105 Daddaoua A,
Puerta V, Requena P, Martinez-Ferez A, Guadix E, de Medina FS, Zarzuelo
A, Suarez MD, Boza JJ,
106 Camuesco D,
Peran L, Comalada M, Nieto A, Di Stasi LC, Rodriguez-Cabezas ME, Concha
A, Zarzuelo A, Galvez J.
107 Rumi G,
Tsubouchi R, Okayama M, Kato S, Mozsik G, Takeuchi K. Protective effect
of lactulose on dextran sulfate
108 Moreau NM,
Martin LJ, Toquet CS, Laboisse CL, Nguyen PG, Siliart BS, Dumon HJ,
Champ MM. Restoration of the
109 Holma R,
Juvonen P, Asmawi MZ, Vapaatalo H, Korpela R. Galacto-oligosaccharides
stimulate the growth of
110 Fukuda M,
Kanauchi O, Araki Y, Andoh A, Mitsuyama K, Takagi K, Toyonaga A, Sata M,
Fujiyama Y, Fukuoka M,
111 Kanauchi O,
Iwanaga T, Andoh A, Araki Y, Nakamura T, Mitsuyama K, Suzuki A, Hibi T,
Bamba T. Dietary fiber fraction
112 Kanauchi O,
Mitsuyama K, Homma T, Takahama K, Fujiyama Y, Andoh A, Araki Y, Suga T,
Hibi T, Naganuma M,
113 Hanai H,
Kanauchi O, Mitsuyama K, Andoh A, Takeuchi K, Takayuki I, Araki Y,
Fujiyama Y, Toyonaga A, Sata M, Kojima
114 Furrie E,
Macfarlane S, Kennedy A, Cummings JH, Walsh SV, O’neil DA, Macfarlane
GT. Synbiotic therapy
115 Lindsay JO,
Whelan K, Stagg AJ, Gobin P, Al-Hassi HO, Rayment N, Kamm MA, Knight SC,
Forbes A. Clinical,
116 Guslandi M,
Giollo P, Testoni PA. A pilot trial of Saccharomyces boulardii in
ulcerative colitis. Eur J Gastroenterol Hepatol
117 Shanahan F,
Guraner F, von Wright A, Vilpponene-Salmela T, O’Donoghue D, Kiely B. A
one year, randomised, double-
118 McCarthy J,
O’Mahony L, Dunne C. An open trial of a novel probiotic as an
alternative to steroids in mild/moderately
119 Gupta P,
Andrew H, Kirschner BS, Guandalini S. Is lactobacillus GG helpful in
children with Crohn’s disease? Results of a
120 Campieri M,
Rizzello F, Venturi A. Combination of antibiotic and probiotic treatment
is efficacious in prophylaxis of post-
121 Marteau P,
Lemann M, Seksik P, Laharie D, Colombel JF, Bouhnik Y, Cadiot G, Soule
JC, Bourreille A, Metman E,
122 Laake KO,
Line PD, Grzyb K, Aamodt G, Aabakken L, Roset A, Hvinden AB, Bakka A,
Eide J, Bjorneklett A, Vatn MH.
123 Mimura T,
Rizzello F, Helwig U, Poggioli G, Schreiber S, Talbot IC, Nicholls RJ,
Gionchetti P, Campieri M, Kamm MA.
S- Editor Wang J L- Editor Wang XL E- Editor Ma WH