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ISSN 1007-9327 CN 14-1219/R  World J Gastroenterol  2006 February 28;12(8):1198-1204

 

Distinct toll-like receptor expression in monocytes and T cells in chronic HCV infection

 

Angela Dolganiuc, Catherine Garcia, Karen Kodys, Gyongyi Szabo

 

 


 


 

Angela Dolganiuc, Catherine Garcia, Karen Kodys, Gyongyi Szabo. Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605-2324, United States

Supported by PHS grant AA12862 (to GS), UMMS Center for AIDS Research Core Facility CFAR (grant 5P30 AI42845), Diabetes Endocrinology Research Center (PHS grant DK32520) and by NIH Summer Research Fellowship (to CG)

Correspondence to: Gyongyi Szabo, MD, PhD, University of Massachusetts Medical School, Department of Medicine, LRB 215, 364 Plantation Street, Worcester, MA 01605, United States. gyongyi.szabo@umassmed.edu

Telephone: +1-508-856-5275     Fax: +1-508-856-4770

Received: 2005-09-14           Accepted: 2005-10-26 

  

Abstract

AIM: Hepatitis C virus often establishes chronic infections. Recent studies suggest that viral and bacterial infections are more common in HCV-infected patients compared to controls. Pathogens are recognized by Toll-like receptors (TLRs) to shape adaptive and innate immune responses.

 

METHODS: In this study, to assess the ability of HCV-infected host to recognize invading pathogens, we investigated Toll-like receptor expression in innate (monocytes) and adaptive (T cells) immune cells by real-time PCR.

 

RESULTS: We determined that RNA levels for TLRs 2, 6. 7, 8, 9 and 10 mRNA levels were upregulated in both monocytes and T cells in HCV-infected patients compared to controls. TLR4 was only upregulated in T lymphocytes, while TLR5 was selectively increased in monocytes of HCV-infected patients. MD-2, a TLR4 co-receptor, was increased in patientsí monocytes and T cells while CD14 and MyD88 were increased only in monocytes.

 

CONCLUSION: Our data reveal novel details on TLR expression that likely relates to innate recognition of pathogens and immune defense in HCV-infected individuals.

 

© 2006 The WJG Press. All rights reserved.

 

Key words: Hepatitis C virus; Toll-like receptors; T cells; Monocytes

 

Dolganiuc A, Garcia C, Kodys K, Szabo G. Distinct toll-like receptor expression in monocytes and T cells in chronic HCV infection. World J Gastroenterol 2006; 12(8): 1198-1204